Soldan S S, Graf M D, Waziri A, Flerlage A N, Robinson S M, Kawanishi T, Leist T P, Lehky T J, Levin M C, Jacobson S
Viral Immunology Section, National Institute of Neurological Disorders and Stroke, Bethesda, Maryland 20892, USA.
J Infect Dis. 1999 Sep;180(3):685-94. doi: 10.1086/314923.
The human T-cell lymphotropic virus type I (HTLV-I) is associated with a chronic, progressive neurological disease known as HTLV-I-associated myelopathy/tropical spastic paraparesis. Screening for HTLV-I involves the detection of virus-specific serum antibodies by EIA and confirmation by Western blot. HTLV-I/II seroindeterminate Western blot patterns have been described worldwide. However, the significance of this blot pattern is unclear. We identified 8 patients with neurological disease and an HTLV-I/II seroindeterminate Western blot pattern, none of whom demonstrated increased spontaneous proliferation and HTLV-I-specific cytotoxic T lymphocyte activity. However, HTLV-I tax sequence was amplified from the peripheral blood lymphocytes of 4 of them. These data suggest that patients with chronic progressive neurological disease and HTLV-I/II Western blot seroindeterminate reactivity may harbor either defective HTLV-I, novel retrovirus with partial homology to HTLV-I, or HTLV-I in low copy number.
人类嗜T细胞病毒I型（HTLV-I）与一种称为HTLV-I相关脊髓病/热带痉挛性截瘫的慢性进行性神经疾病有关。HTLV-I的筛查包括通过酶免疫测定法检测病毒特异性血清抗体，并通过蛋白质印迹法进行确认。HTLV-I/II血清学不确定的蛋白质印迹模式已在全球范围内被描述。然而，这种印迹模式的意义尚不清楚。我们鉴定出8例患有神经疾病且蛋白质印迹模式为HTLV-I/II血清学不确定的患者，他们均未表现出自发性增殖增加和HTLV-I特异性细胞毒性T淋巴细胞活性。然而，其中4例患者的外周血淋巴细胞中扩增出了HTLV-I tax序列。这些数据表明，患有慢性进行性神经疾病且蛋白质印迹反应为HTLV-I/II血清学不确定的患者可能携带缺陷型HTLV-I、与HTLV-I有部分同源性的新型逆转录病毒或低拷贝数的HTLV-I。
