运用逆转录聚合酶链反应检测增殖性玻璃体视网膜病变中浸润细胞内细胞因子mRNA的产生
Detection of cytokine mRNA production in infiltrating cells in proliferative vitreoretinopathy using reverse transcription polymerase chain reaction.
作者信息
El-Ghrably I A, Dua H S, Orr G M, Fischer D, Tighe P J
机构信息
Department of Ophthalmology, University of Nottingham.
出版信息
Br J Ophthalmol. 1999 Nov;83(11):1296-9. doi: 10.1136/bjo.83.11.1296.
AIMS
To determine whether the infiltrating cells in the vitreous and subretinal fluid of patients with proliferative vitreoretinopathy (PVR) express messenger RNA for various cytokines found in this condition.
METHODS
The presence of mRNA coding for HPRT, IL-6, IL-1beta, IL-8, and TNFalpha was investigated in 20 vitreous and subretinal fluid (SRF) samples from patients with PVR by reverse transcriptase polymerase chain reaction (RT-PCR). 16 samples from patients with retinal detachment and macular holes were used as controls.
RESULTS
HPRT was detected in all samples of PVR and in 11 (69%) control cases. Patients with PVR demonstrated mRNA for the cytokines tested more often than controls. The difference was statistically significant.
CONCLUSION
The presence of mRNA encoding for IL-6, IL-1beta, IL-8, and TNFalpha is significantly detected by RT-PCR in vitreous and SRF samples of patients with PVR, indicating local production of these cytokines by vitreous and SRF cells.
目的
确定增殖性玻璃体视网膜病变（PVR）患者玻璃体液和视网膜下液中的浸润细胞是否表达该疾病中发现的各种细胞因子的信使核糖核酸。
方法
通过逆转录聚合酶链反应（RT-PCR）研究了20例PVR患者玻璃体液和视网膜下液（SRF）样本中编码次黄嘌呤磷酸核糖转移酶（HPRT）、白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β）、白细胞介素-8（IL-8）和肿瘤坏死因子α（TNFα）的信使核糖核酸的存在情况。将16例视网膜脱离和黄斑裂孔患者的样本用作对照。
结果
在所有PVR样本以及11例（69%）对照病例中均检测到HPRT。PVR患者检测到所测试细胞因子信使核糖核酸的频率高于对照。差异具有统计学意义。
结论
通过RT-PCR在PVR患者的玻璃体液和SRF样本中显著检测到编码IL-6、IL-1β、IL-8和TNFα的信使核糖核酸的存在，表明这些细胞因子由玻璃体液和SRF细胞在局部产生。
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