Delori F C, Fleckner M R, Goger D G, Weiter J J, Dorey C K
Schepens Eye Research Institute, Boston, Massachusetts 02114, USA.
Invest Ophthalmol Vis Sci. 2000 Feb;41(2):496-504.
To determine whether drusen in patients with age-related maculopathy and macular degeneration (ARM/AMD) are associated with focal changes in retinal pigment epithelium (RPE) lipofuscin fluorescence.
A new autofluorescence imaging device was used to study lipofuscin distribution associated with individual drusen in 20 patients with ARM/AMD. Paired monochromatic and autofluorescence fundus images were used for detailed analysis of the topography of autofluorescence at specific sites containing drusen. In four eyes, image analysis was used to compare the spatial distribution of the autofluorescence with the location of drusen and to quantify the autofluorescence distribution over individual drusen (54 drusen). REsuLTs. A specific pattern of autofluorescence was frequently found to be spatially associated with hard drusen and soft drusen between 60 and 175 microm in size. The pattern is characterized by a central area of decreased autofluorescence surrounded, in most cases, by an annulus of increased autofluorescence. The location of this pattern was highly correlated with the position of individual distinct drusen. The central low autofluorescence focus was on average 16% below the surrounding background, and the annulus, when present, was on average 6% more fluorescent than the background. Soft drusen larger than 175 microm and confluent soft drusen show either multifocal areas of low autofluorescence or a more heterogeneous distribution. CONCLUSIoNs. Autofluorescence imaging permits measurement of RPE lipofuscin at specific sites. RPE overlying drusen have altered autofluorescence, suggesting changes in RPE health.
确定年龄相关性黄斑病变和黄斑变性（ARM/AMD）患者的玻璃疣是否与视网膜色素上皮（RPE）脂褐素荧光的局灶性改变相关。
使用一种新的自发荧光成像设备研究20例ARM/AMD患者中与单个玻璃疣相关的脂褐素分布。配对的单色和自发荧光眼底图像用于详细分析含有玻璃疣的特定部位的自发荧光地形图。在4只眼中，使用图像分析比较自发荧光的空间分布与玻璃疣的位置，并量化单个玻璃疣（54个玻璃疣）上的自发荧光分布。结果。经常发现一种特定的自发荧光模式在空间上与大小在60至175微米之间的硬性玻璃疣和软性玻璃疣相关。该模式的特征是自发荧光降低的中心区域，在大多数情况下，周围是自发荧光增加的环。这种模式的位置与单个明显玻璃疣的位置高度相关。中心低自发荧光焦点平均比周围背景低16%，环（如果存在）平均比背景荧光高6%。大于175微米的软性玻璃疣和融合性软性玻璃疣显示出多灶性低自发荧光区域或更不均匀的分布。结论。自发荧光成像允许在特定部位测量RPE脂褐素。覆盖玻璃疣的RPE有改变的自发荧光，提示RPE健康状况的改变。
