Nilsson I, Lindgren S, Eriksson S, Wadström T
Department of Infectious Diseases and Medical Microbiology, University of Lund, Sweden.
Gut. 2000 Mar;46(3):410-4. doi: 10.1136/gut.46.3.410.
Bile tolerant helicobacter species such as H hepaticus and H bilis have frequently been reported to cause hepatitis in mice and other rodents.
To investigate the possible pathogenic role of these and other helicobacter species in chronic liver disease in humans.
Serum samples from 144 patients with various chronic liver diseases, 30 patients with primary sclerosing cholangitis (PSC), and 48 healthy blood donors were analysed for antibodies against H hepaticus murine strain CCUG 33637 and H pylori strain CCUG 17874. Cell surface proteins of H hepaticus were extracted by acid glycine buffer and used in an enzyme immunoassay (EIA) and immunoblot (IB).
56 of 144 (39%) patients with chronic liver diseases and six of 30 (20%) with PSC showed increased antibody concentrations in the H hepaticus EIA; in the H pylori EIA the numbers were 58% and 13% respectively. Compared with the healthy blood donors the antibody reactivity against the two helicobacter species was not increased (46% and 48% respectively). Patient serum samples retested by the H hepaticus EIA after absorption with sonicated H pylori cells remained positive in 12 of 37 (33%) serum samples. Distinct antibody reactivity to 55-65 kDa proteins was observed by H hepaticus IB, after the absorption step, and was considered specific for H hepaticus. These 12 serum samples were from patients with chronic alcoholic liver disease.
Antibodies to H hepaticus, often cross reacting with H pylori, occur frequently in patients with chronic liver diseases, with no clear cut relation to specific diagnostic groups. The pathogenic significance of these findings is not known.
诸如肝螺杆菌和胆汁螺杆菌等耐胆汁的螺杆菌属物种经常被报道可在小鼠和其他啮齿动物中引发肝炎。
研究这些及其他螺杆菌属物种在人类慢性肝病中可能的致病作用。
分析了144例各种慢性肝病患者、30例原发性硬化性胆管炎（PSC）患者以及48名健康献血者的血清样本，检测其针对肝螺杆菌鼠株CCUG 33637和幽门螺杆菌株CCUG 17874的抗体。用酸性甘氨酸缓冲液提取肝螺杆菌的细胞表面蛋白，并用于酶免疫测定（EIA）和免疫印迹（IB）。
144例慢性肝病患者中有56例（39%）以及30例PSC患者中有6例（20%）在肝螺杆菌EIA中显示抗体浓度升高；在幽门螺杆菌EIA中，这一比例分别为58%和13%。与健康献血者相比，针对这两种螺杆菌的抗体反应性并未增加（分别为46%和48%）。用超声处理过的幽门螺杆菌细胞吸收后，再通过肝螺杆菌EIA重新检测的患者血清样本中，37份血清样本中有12份（33%）仍为阳性。在吸收步骤后，通过肝螺杆菌免疫印迹观察到对55 - 65 kDa蛋白有明显的抗体反应性，并被认为是肝螺杆菌特有的。这12份血清样本来自慢性酒精性肝病患者。
慢性肝病患者中经常出现与幽门螺杆菌常有交叉反应的抗肝螺杆菌抗体，与特定诊断组无明确关系。这些发现的致病意义尚不清楚。
