McGeoch D, Fellner P, Newton C
Proc Natl Acad Sci U S A. 1976 Sep;73(9):3045-9. doi: 10.1073/pnas.73.9.3045.
The genomic RNA of the avian influenza A virus, fowl plague, was fractionated into eight species by electrophoresis in polyacrylamide-agarose gels containing 6 M urea. The separated 32P-labeled RNA species were characterized by digestion with RNase T1 and fractionation of the resulting oligonucleotides by two-dimensional gel electrophoresis; this demonstrated that each species has a distinct nucleotide sequence. A tentative correlation of each genome RNA species with the virus protein that it encodes was made.
通过在含有6M尿素的聚丙烯酰胺-琼脂糖凝胶中进行电泳，将禽甲型流感病毒（鸡瘟）的基因组RNA分离成八个种类。分离出的32P标记的RNA种类通过用核糖核酸酶T1消化并通过二维凝胶电泳对所得寡核苷酸进行分级分离来表征；这表明每个种类都有独特的核苷酸序列。对每个基因组RNA种类与其编码的病毒蛋白进行了初步关联。
