用于检测尿液样本中军团菌抗原的快速免疫层析测定法的评估。
Evaluation of a rapid immunochromatographic assay for the detection of Legionella antigen in urine samples.
作者信息
Domínguez J, Galí N, Matas L, Pedroso P, Hernández A, Padilla E, Ausina V
机构信息
Servicio de Microbiología, Hospital Universitari Germans Trias i Pujol, Facultad de Medicina de la Universitat Autònoma de Barcelona, Spain.
出版信息
Eur J Clin Microbiol Infect Dis. 1999 Dec;18(12):896-8. doi: 10.1007/s100960050427.
A new immunochromatographic membrane assay for detecting Legionella pneumophila serogroup 1 antigen in urine samples (Binax Now Legionella Urinary Antigen Test; Binax, USA) was evaluated. Its sensitivity, specificity and level of agreement with the Binax enzyme immunoassay were compared using nonconcentrated and concentrated urine samples. The overall agreement between the two tests was 98.1%; the specificity of both was 100%. The sensitivity of the immunochromatographic assay was 55.5% in nonconcentrated urine and 97.2% in concentrated urine in comparison with the enzyme immunoassay, using concentrated urine as the reference test. This immunochromatographic assay screens successfully for Legionella pneumophila serogroup 1 soluble antigen in concentrated urine samples.
对一种用于检测尿液样本中嗜肺军团菌血清1型抗原的新型免疫层析膜检测法（Binax Now军团菌尿液抗原检测；美国Binax公司）进行了评估。使用未浓缩和浓缩尿液样本，比较了其与Binax酶免疫测定法的敏感性、特异性和一致性水平。两种检测方法的总体一致性为98.1%；两者的特异性均为100%。以浓缩尿液作为参考检测法，与酶免疫测定法相比，免疫层析检测法在未浓缩尿液中的敏感性为55.5%，在浓缩尿液中的敏感性为97.2%。这种免疫层析检测法能成功筛查浓缩尿液样本中的嗜肺军团菌血清1型可溶性抗原。
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