用于检测浓缩和非浓缩尿液样本中嗜肺军团菌1型抗原的放射免疫分析试剂盒和酶免疫分析试剂盒的比较
Comparison of radioimmunoassay and enzyme immunoassay kits for detection of Legionella pneumophila serogroup 1 antigen in both concentrated and nonconcentrated urine samples.
作者信息
Domínguez J A, Matas L, Manterola J M, Blavia R, Sopena N, Belda F J, Padilla E, Giménez M, Sabrià M, Morera J, Ausina V
机构信息
Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Facultad de Medicina de la Universidad Autónoma de Barcelona, Spain.
出版信息
J Clin Microbiol. 1997 Jun;35(6):1627-9. doi: 10.1128/jcm.35.6.1627-1629.1997.
Abstract
We evaluated a commercial enzyme immunoassay (EIA) kit for detection of Legionella pneumophila serogroup 1 soluble antigen by comparing it to radioimmunoassay (RIA), using both concentrated and nonconcentrated urine samples. The sensitivity of EIA was 67.4% in nonconcentrated urine samples and 82.6% in concentrated urine samples. The sensitivity of RIA was 60.9% and 84.8% in nonconcentrated and concentrated urine samples, respectively. Our study indicates that the sensitivity and specificity of EIA are comparable to those of RIA, and that concentrating the antigen by selective ultrafiltration increases sensitivity for both EIA and RIA, with no significant decrease in specificity.
摘要
我们通过将一种用于检测嗜肺军团菌血清1型可溶性抗原的商用酶免疫测定（EIA）试剂盒与放射免疫测定（RIA）进行比较，使用浓缩和未浓缩的尿液样本对其进行了评估。EIA在未浓缩尿液样本中的灵敏度为67.4%，在浓缩尿液样本中的灵敏度为82.6%。RIA在未浓缩和浓缩尿液样本中的灵敏度分别为60.9%和84.8%。我们的研究表明，EIA的灵敏度和特异性与RIA相当，并且通过选择性超滤浓缩抗原可提高EIA和RIA的灵敏度，而特异性没有显著降低。
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