Luzzati A L, Taussig M J, Meo T, Pernis B
J Exp Med. 1976 Sep 1;144(3):573-85. doi: 10.1084/jem.144.3.573.
A culture system is descirbed which provides adequate conditions for in vitro immunization of humand peripheral blood lymphocytes to heterologous erythrocytes. Making use of this method we could obtain, with a number of different donors, an antibody response which peaked at about day 8 of culture with 30-300 plaque-forming cells (PFC) per 10(6) input lymphocytes. However, in a number of experiments poor or negative results were obtained, even with donors that had previously given good response. This variability in the results was shown not to be due to a too low number of precursor cells present in the blood and could be overcome by treating the cells, before initiation of the culture, with a factor produced by mouse T cells educated to sheep erythrocytes (SRBC). Under these conditions a PFC responce was obtained which peaked at about day 8 and which in some experiments could be as high as 20,000 PFC per 10(6) input lymphocytes. Paralleling the increase in PFC was an increase in cell number. The cells recovered from the treated cultures were at all times more numerous than in the nontreated cultures. The height of both the proliferative and antibody-producing responses varied from experiment to experiment, a higher proliferative response, accompanying a higher PFC response. Although the mechanisms that are at the basis of the antibody response in vitro described in this paper still need to be clarified, this system may become a useful tool in studying the immune response in man.
本文描述了一种培养系统，该系统为人类外周血淋巴细胞体外免疫异源红细胞提供了适宜条件。利用这一方法，我们从多个不同供体获得了抗体反应，该反应在培养约第8天达到峰值，每10⁶个输入淋巴细胞中有30 - 300个空斑形成细胞（PFC）。然而，在一些实验中，即使是之前反应良好的供体，也得到了较差或阴性的结果。结果的这种变异性并非由于血液中前体细胞数量过少所致，并且可以通过在培养开始前用经绵羊红细胞（SRBC）致敏的小鼠T细胞产生的一种因子处理细胞来克服。在这些条件下获得了PFC反应，该反应在约第8天达到峰值，在一些实验中每10⁶个输入淋巴细胞中PFC数量可高达20,000个。与PFC增加平行的是细胞数量的增加。从经处理的培养物中回收的细胞在任何时候都比未处理的培养物中的细胞多。增殖反应和抗体产生反应的强度在不同实验中有所不同，增殖反应越高，PFC反应也越高。尽管本文所述体外抗体反应的基础机制仍需阐明，但该系统可能成为研究人类免疫反应的有用工具。
