Jay A W
Biophys J. 1975 Mar;15(3):205-22. doi: 10.1016/S0006-3495(75)85812-7.
The effects of albumin on the geometry of human erythrocytes have been studied. Individual red cells, hanging on edge from coverslips were photographed. Enlarged cell profiles were digitized using a Gradicon digitizer (Instronics Ltd., Stittsville, Ontario). Geometric parameters including diameter, area, volume, minimum cylindrical diameter, sphericity index, swelling index, maximum and minimum cell thickness, were calculated for each cell using a CDC 6400 computer. Maximum effect of human serum albumin was reached at about 1 g/liter. Studies of cell populations showed decreases in mean cell diameter of up to 6%, area 6%, and volume 15%, varying from sample to sample. The thickness of the rim was increased while that at the dimple was decreased. Studies of single cells showed that area and volume changes do not occur equally in all cells. Cells with lower sphericity indices showed larger effects. In the presence of albumin, up to 50% of the cells assumed cup-shapes (stomatocytes). These cells had smaller volumes but the same area as biconcave cells. Mechanical agitation could reversibly induce biconcave cells to assume cup shapes without area or volume changes. Experiments with de-fatted human albumins showed that the presence of bound fatty acids in varying concentrations does not alter the observed effects. Bovine serum albumin has similar effects on human erythrocytes as human serum albumin.
研究了白蛋白对人红细胞几何形状的影响。对单个挂在盖玻片边缘的红细胞进行拍照。使用Gradicon数字化仪（Instronics有限公司，安大略省斯蒂茨维尔）对放大的细胞轮廓进行数字化处理。使用CDC 6400计算机为每个细胞计算包括直径、面积、体积、最小圆柱直径、球形指数、肿胀指数、最大和最小细胞厚度在内的几何参数。人血清白蛋白的最大作用在约1克/升时达到。对细胞群体的研究表明，平均细胞直径最多减少6%，面积减少6%，体积减少15%，不同样本有所差异。边缘厚度增加，而凹陷处厚度减少。对单个细胞的研究表明，并非所有细胞的面积和体积变化都相同。球形指数较低的细胞受到的影响更大。在白蛋白存在的情况下，高达50%的细胞呈现杯状（口形细胞）。这些细胞的体积较小，但面积与双凹细胞相同。机械搅拌可使双凹细胞可逆地呈现杯状，而面积和体积不变。用脱脂人白蛋白进行的实验表明，不同浓度结合脂肪酸的存在不会改变观察到的效果。牛血清白蛋白对人红细胞的作用与人血清白蛋白相似。
