通过白细胞游走抑制试验测定感染梅毒螺旋体的家兔的细胞反应。
Cell response in rabbits infected with T. pallidum as measured by the leucocyte migration inhibition test.
作者信息
Wicher V, Wicher K
出版信息
Br J Vener Dis. 1975 Aug;51(4):240-5. doi: 10.1136/sti.51.4.240.
Leucocyte migration in agarose was used to examine cell response in 48 rabbits infected with the Nichols strain of T. pallidum for 1 week to 16 months. The response of the peripheral leucocytes to T. pallidum antigen (TpAg), 3 and 15 mug/ml., Reiter antigen (RAg) 6 mug./ml., and VDRL reagent 1:500 was examined. The cells in the presence of RAg, TpAg--3 mug./ml.--and the VDRL reagent demonstrated a biphasic response; in the early stage of infection (until the 4th week) stimulation of leucocyte migration and in the later stage of disease inhibition of leucocyte migration was observed. The higher concentration of TpAg--15 mug./ml.--caused only inhibition of leucocyte migration. The leucocyte response in vitro could not be confirmed by intradermal skin tests with 5 x 10(7) heat-killed T. pallidum. No correlation between the cell response and the treponemal antibodies was observed.
采用琼脂糖中白细胞迁移试验，检测48只感染梅毒螺旋体Nichols株1周~16个月的家兔的细胞反应。检测外周血白细胞对梅毒螺旋体抗原（TpAg）（3μg/ml和15μg/ml）、Reiter抗原（RAg）（6μg/ml）及VDRL试剂（1:500）的反应。在RAg、3μg/ml TpAg及VDRL试剂存在的情况下，细胞呈现双相反应；在感染早期（至第4周），白细胞迁移受到刺激，而在疾病后期则观察到白细胞迁移受到抑制。较高浓度的TpAg（15μg/ml）仅引起白细胞迁移抑制。用5×10⁷热灭活梅毒螺旋体进行皮内皮肤试验，未能证实体外白细胞反应。未观察到细胞反应与梅毒抗体之间的相关性。
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