MacPherson B R, Albertini R J, Beeken W L
Gut. 1976 Feb;17(2):100-6. doi: 10.1136/gut.17.2.100.
An investigation of immunological parameters was conducted in 38 patients with Crohn's disease. The immunological tests employed included skin tests with dinitrochlorobenzene and a battery of common skin test antigens, lymphocyte transformation with phytohaemagglutinin and pokeweed mitogen, serum immunoglobulins, and absolute lymphocyte counts. Crohn's disease patients were divided into two groups, those treated with immunosuppressive drugs and those not receiving immunosuppressive medications. The latter group was subdivided into patients with active and inactive disease. Immunosuppressed patients with Crohn's disease did not develop sensitivity to dinitrochlorobenzene and had mildly depressed skin test reactivity to common skin test procedures. Non-immunosuppressed patients with active Crohn's disease also reacted less frequently to common skin test antigens, but 16 of 17 such patients developed sensitivity to dinitrochlorobenzene. Lymphocyte transformation with phytohaemagglutinin and pokeweed mitogen was normal in all groups of patients with Crohn's disease. However, when suboptimal incubation periods were used with phytohaemagglutinin stimulation, there was a significant difference between Crohn's disease patients and controls. Serum immunoglobulin levels and absolute lymphocyte counts were normal in all Crohn's disease patients. We conclude that immunity in Crohn's disease is qualitatively normal.
对38例克罗恩病患者进行了免疫参数调查。所采用的免疫测试包括用二硝基氯苯和一系列常见皮肤试验抗原进行皮肤试验、用植物血凝素和商陆有丝分裂原进行淋巴细胞转化试验、血清免疫球蛋白检测以及淋巴细胞绝对计数。克罗恩病患者被分为两组，一组接受免疫抑制药物治疗，另一组未接受免疫抑制药物治疗。后一组又细分为疾病活动期和非活动期患者。接受免疫抑制治疗的克罗恩病患者对二硝基氯苯不产生敏感性，对常见皮肤试验程序的皮肤试验反应性轻度降低。患有活动期克罗恩病的未接受免疫抑制治疗的患者对常见皮肤试验抗原的反应也较少，但17例此类患者中有16例对二硝基氯苯产生敏感性。在所有克罗恩病患者组中，用植物血凝素和商陆有丝分裂原进行的淋巴细胞转化试验结果均正常。然而，当用次优孵育期进行植物血凝素刺激时，克罗恩病患者与对照组之间存在显著差异。所有克罗恩病患者的血清免疫球蛋白水平和淋巴细胞绝对计数均正常。我们得出结论，克罗恩病患者的免疫力在性质上是正常的。
