偶然分枝杆菌复合种内的遗传异质性：鉴定的基因型标准。
Genetic heterogeneity within Mycobacterium fortuitum complex species: genotypic criteria for identification.
作者信息
Kirschner P, Kiekenbeck M, Meissner D, Wolters J, Böttger E C
机构信息
Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany.
出版信息
J Clin Microbiol. 1992 Nov;30(11):2772-5. doi: 10.1128/jcm.30.11.2772-2775.1992.
Abstract
A 1.5-kb segment of the DNA that encodes 16S rRNA was amplified by polymerase chain reaction, and 880 nucleotide positions were determined from each of the described biovariants of Mycobacterium fortuitum. Signature sequences which allow rapid identification of M. fortuitum strains at the biovariant level are described. Our data demonstrate a close phylogenetic relationship between Mycobacterium senegalense and M. fortuitum and indicate that the described biovariants of M. fortuitum represent genetically distinct taxa.
摘要
通过聚合酶链反应扩增出编码16S rRNA的1.5kb DNA片段，并从所述偶然分枝杆菌的每个生物变种中确定880个核苷酸位置。描述了可在生物变种水平上快速鉴定偶然分枝杆菌菌株的特征序列。我们的数据表明塞内加尔分枝杆菌与偶然分枝杆菌之间存在密切的系统发育关系，并表明所述偶然分枝杆菌的生物变种代表了遗传上不同的分类群。
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