Ross B C, Jackson K, Yang M, Sievers A, Dwyer B
Clinical Pathology Laboratory, Fairfield Infectious Diseases Hospital, Victoria, Australia.
J Clin Microbiol. 1992 Nov;30(11):2930-3. doi: 10.1128/jcm.30.11.2930-2933.1992.
To assess the usefulness of a specific DNA probe for Mycobacterium kansasii, 105 isolates from Australia, Belgium, Japan, South Africa, and Switzerland were collected and analyzed. Twenty of these isolates were probe negative, of which 18 were from Belgium and Switzerland. Analysis of all isolates by Southern blot hybridization indicated a lack of variability among probe-positive isolates, while probe-negative isolates were clearly distinct and showed greater diversity. Sequence analysis of the 250 nucleotides at the 5' end of the 16S rRNA gene revealed that 19 of the 20 probe-negative isolates had a sequence different from that of M. kansasii. A total of five nucleotide differences were present in a cluster consisting of two nucleotide deletions and three nucleotide substitutions. These results suggest the existence of a genetic subspecies of M. kansasii.
为评估一种针对堪萨斯分枝杆菌的特定DNA探针的实用性，收集并分析了来自澳大利亚、比利时、日本、南非和瑞士的105株分离株。其中20株分离株探针检测呈阴性，其中18株来自比利时和瑞士。通过Southern印迹杂交对所有分离株进行分析表明，探针阳性分离株之间缺乏变异性，而探针阴性分离株明显不同且表现出更大的多样性。对16S rRNA基因5'端250个核苷酸的序列分析显示，20株探针阴性分离株中有19株的序列与堪萨斯分枝杆菌不同。在一个由两个核苷酸缺失和三个核苷酸替换组成的簇中总共存在五个核苷酸差异。这些结果提示存在堪萨斯分枝杆菌的一个遗传亚种。
