首次从土耳其蓖麻硬蜱中分离并鉴定伯氏疏螺旋体狭义种菌株。

First isolation and characterization of Borrelia burgdorferi sensu lato strains from Ixodes ricinus ticks in Turkey.

作者信息

Güner Ece S, Hashimoto Naoya, Takada Nobuhiro, Kaneda Kazuhide, Imai Yasuyuki, Masuzawa Toshiyuki

机构信息

Medical School, Yeditepe University, Istanbul, Turkey 2Department of Microbiology and COE Program in the 21st Century, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, Japan 3Department of Immunology and Medical Zoology, Fukui Medical University, Matsuoka, Fukui, Japan 4Department of Food Science, Shizuoka Eiwa Gakuen University Junior College, Shizuoka, Japan.

出版信息

J Med Microbiol. 2003 Sep;52(Pt 9):807-813. doi: 10.1099/jmm.0.05205-0.

DOI:10.1099/jmm.0.05205-0

PMID:12909659

Abstract

In order to investigate the presence and prevalence of Lyme borreliosis (Lyme disease) Borrelia species, 312 unfed ticks were collected by flagging at a woodland area in Trakya, in the European side of Turkey, in May 2002. Twelve of 299 Ixodes ricinus ticks were infected with Borrelia spp., as determined by cultivation in BSK medium (prevalence rate 4.0 %). Ten pure cultures were subjected to further characterization by sequencing analysis of the 5S-23S rDNA intergenic spacer, 16S rDNA and flagellin gene. One isolate of Borrelia burgdorferi sensu stricto, two of Borrelia garinii (Eurasian type), two of Borrelia afzelii, four of Borrelia lusitaniae and one of Borrelia valaisiana were identified. However, no Asian-type B. garinii was found. Interestingly, all Borrelia species that are known to be carried by I. ricinus were discovered among the 10 isolates. These results provide the first evidence for the existence of the Lyme borreliosis agent in Turkey.

摘要

为了调查莱姆病疏螺旋体的存在情况和流行程度，2002年5月，在土耳其欧洲部分的特拉布宗一个林区，通过挥旗法收集了312只未进食的蜱。在299只蓖麻硬蜱中，有12只感染了疏螺旋体属，这是通过在BSK培养基中培养确定的（流行率为4.0%）。对10个纯培养物通过5S - 23S rDNA基因间隔区、16S rDNA和鞭毛蛋白基因的测序分析进行进一步鉴定。鉴定出1株狭义伯氏疏螺旋体、2株加氏疏螺旋体（欧亚型）、2株阿氏疏螺旋体、4株葡萄牙疏螺旋体和1株瓦莱疏螺旋体。然而，未发现亚洲型加氏疏螺旋体。有趣的是，在这10株分离株中发现了所有已知由蓖麻硬蜱携带的疏螺旋体物种。这些结果为土耳其存在莱姆病病原体提供了首个证据。