Postiglione L, Di Domenico G, Ramaglia L, Montagnani S, Salzano S, Di Meglio F, Sbordone L, Vitale M, Rossi G
Dept. of Cellular and Molecular Biology and Pathology, "L. Califano", Naples, Italy.
J Dent Res. 2003 Sep;82(9):692-6. doi: 10.1177/154405910308200907.
Surface properties may affect the clinical outcome of titanium dental implants. The aim of the present study was to investigate the effects of 3 different titanium surfaces-smooth (S), sandblasted (SB), and titanium plasma-sprayed (TPS)-on proliferation, differentiation, and apoptosis of human osteoblast-like cells, SaOS-2. Cell proliferation was significantly (p < 0.05) higher on the S surface, and synthesis of extracellular matrix proteins was more abundant on TPS and SB than on S surfaces. Analysis of integrin receptors showed a higher expression of alpha2, alpha5, alphaVbeta3, and ss1 on TPS as compared with SB and S surfaces. An increase in alkaline phosphatase activity was detected only on SB and TPS surfaces. Analysis of cell apoptosis did not demonstrate any significant difference among the 3 different surfaces. The results indicate that titanium surface topography affects proliferation and differentiation of osteoblast-like SaOS-2 cells, suggesting that surface properties might be important for bone response around dental implants in vivo.
表面特性可能会影响钛牙种植体的临床效果。本研究的目的是调查3种不同钛表面——光滑表面（S）、喷砂表面（SB）和钛等离子喷涂表面（TPS）——对人成骨样细胞SaOS-2增殖、分化和凋亡的影响。在S表面细胞增殖显著更高（p < 0.05），并且在TPS和SB表面细胞外基质蛋白的合成比在S表面更丰富。整合素受体分析显示，与SB和S表面相比，TPS表面上的α2、α5、αVβ3和β1表达更高。仅在SB和TPS表面检测到碱性磷酸酶活性增加。细胞凋亡分析未显示3种不同表面之间有任何显著差异。结果表明，钛表面形貌影响成骨样SaOS-2细胞的增殖和分化，这表明表面特性可能对体内牙种植体周围的骨反应很重要。
