Osathanon Thanaphum, Bespinyowong Kritchai, Arksornnukit Mansuang, Takahashi Hidekazu, Pavasant Prasit
Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Henri-Dunant Rd., Pathumwan, Bangkok, 10330, Thailand.
J Mater Sci Mater Med. 2006 Jul;17(7):619-25. doi: 10.1007/s10856-006-9224-8.
The purpose of this study was to compare the early response of human osteoblast-like cells (SaOS-2) on commercially pure titanium (cpTi) and titanium-6-aluminium-7-niobium (Ti-6Al-7Nb) using glass slide as a control. In terms of cell attachment, no significance was observed when cells were seeded on the materials. However, morphological analysis by scanning electron microscope revealed that cells on Ti-6Al-7Nb showed better spreading after 4 hrs. After 48 hrs, both Western analysis and reverse transcription polymerase chain reaction analyses showed that cells cultured on Ti-6Al-7Nb synthesized a higher amount of fibronectin and osteopontin as compared to cells seeded on cpTi or on glass slide. These results suggest that Ti-6Al-7Nb possess a good potential to support SaOS-2 cells on spreading and fibronectin and osteopontin synthesis, therefore, this material may be one of a candidate material used in implant dentistry.
本研究的目的是使用载玻片作为对照，比较人成骨样细胞（SaOS-2）在商业纯钛（cpTi）和钛-6-铝-7-铌（Ti-6Al-7Nb）上的早期反应。就细胞附着而言，当细胞接种在材料上时未观察到显著差异。然而，扫描电子显微镜的形态学分析显示，在Ti-6Al-7Nb上的细胞在4小时后铺展得更好。48小时后，蛋白质免疫印迹分析和逆转录聚合酶链反应分析均表明，与接种在cpTi或载玻片上的细胞相比，在Ti-6Al-7Nb上培养的细胞合成了更多的纤连蛋白和骨桥蛋白。这些结果表明，Ti-6Al-7Nb在支持SaOS-2细胞铺展以及纤连蛋白和骨桥蛋白合成方面具有良好的潜力，因此，这种材料可能是口腔种植学中使用的候选材料之一。
