Springer M S, Davidson E H, Britten R J
Kerckhoff Marine Laboratory, California Institute of Technology, Corona del Mar 92625.
J Mol Evol. 1992 May;34(5):379-82. doi: 10.1007/BF00162994.
Measurements are reported of the thermal stability of DNA heteroduplexes between clones of the eta-globin pseudogene from a variety of primates. The known sequences of this 7.1-kb region differ from each over a range from 1.6% for human versus chimp to nearly 12% for human versus spider monkey. Thermal stability was determined by standard hydroxyapatite thermal elution, and the results show a precisely linear decrease in thermal stability with divergence. The slope of the regression line is 1.18% sequence divergence per degree centigrade reduction in thermal stability.
报道了来自多种灵长类动物的η-珠蛋白假基因克隆之间DNA异源双链体的热稳定性测量结果。这个7.1千碱基区域的已知序列在不同物种间存在差异，人类与黑猩猩之间的差异为1.6%，而人类与蜘蛛猴之间的差异接近12%。通过标准的羟基磷灰石热洗脱法测定热稳定性，结果显示热稳定性随序列差异呈精确的线性下降。回归线的斜率为热稳定性每降低一摄氏度，序列差异增加1.18%。
