Lung M L, Lam W P, Chan K H, Li S, Sham J, Choy D
Department of Microbiology, Queen Mary Hospital, University of Hong Kong.
Int J Cancer. 1992 Sep 9;52(2):174-7. doi: 10.1002/ijc.2910520203.
By means of a PCR assay, EBV was demonstrated directly in peripheral blood of previously infected individuals. The virus was detected in approximately 80% of specimens from EBV-seropositive individuals, but not in cord-blood lymphocytes by this method. When virus present in peripheral blood was compared to that observed directly in NPC biopsies or throat washings, it was distinct from that seen in biopsies in 4/15 cases (27%) and from that seen in throat washes in 1/22 cases (5%). The throat-wash virus differed from the biopsy virus in 3/20 cases (15%). The prototype F virus was found in 7/10 LCLs (70%) established from NPC patients' peripheral blood, but was only detected in 2/9 specimens (22%) directly analyzed by the PCR assay. This finding suggests selective isolation of prototype F EBV in spontaneous LCLs established from NPC patients.
通过聚合酶链反应（PCR）检测法，在既往感染个体的外周血中直接检测到了EB病毒。用此方法在约80%的EB病毒血清阳性个体的标本中检测到了该病毒，但在脐带血淋巴细胞中未检测到。当将外周血中存在的病毒与在鼻咽癌活检组织或咽喉冲洗液中直接观察到的病毒进行比较时，在4/15例（27%）中其与活检组织中所见的病毒不同，在1/22例（5%）中与咽喉冲洗液中所见的病毒不同。咽喉冲洗液中的病毒在3/20例（15%）中与活检组织中的病毒不同。在从鼻咽癌患者外周血建立的10个淋巴母细胞样细胞系（LCL）中有7个（70%）发现了原型F病毒，但通过PCR检测法直接分析时仅在9个标本中的2个（22%）中检测到。这一发现提示，在从鼻咽癌患者建立的自发LCL中可选择性分离出原型F型EB病毒。
