Han T
Clin Exp Immunol. 1976 Aug;25(2):338-41.
"One-way" mixed lymphocyte reaction was not enhanced when the cells were allowed to be exposed to acetylcholine or carbamylcholine at a concentration of 0.01-1.0mumole/ml during the entire period of culture. It was thought to be due to cytotoxicity effect of these agents on responding cells. When the responding cells were incubated with cholinergic agents for 1 hr prior to washing the cells and setting up the mixed cell culture, "one-way" mixed lymphocyte reaction was enhanced. However, when the stimulating cells were similarly treated with either agent, no enhancement of "one-way" mixed lymphocyte reaction was observed. These observations indicate that cholinergic agent induces enhancement of mixed lymphocyte reaction by affecting only the responding cells and not the stimulating cells.
在整个培养期间，当细胞暴露于浓度为0.01 - 1.0微摩尔/毫升的乙酰胆碱或氨甲酰胆碱时，“单向”混合淋巴细胞反应并未增强。据认为，这是由于这些试剂对反应细胞的细胞毒性作用所致。当在洗涤细胞并建立混合细胞培养之前，将反应细胞与胆碱能试剂孵育1小时，“单向”混合淋巴细胞反应增强。然而，当用任何一种试剂对刺激细胞进行类似处理时，未观察到“单向”混合淋巴细胞反应增强。这些观察结果表明，胆碱能试剂仅通过影响反应细胞而非刺激细胞来诱导混合淋巴细胞反应增强。
