流感病毒在组织培养中产生的一种非传染性细胞病变效应，伴有非感染性血凝素的形成。
A non-transmissible cytopathogenic effect of influenza virus in tissue culture accompanied by formation of non-infectious hemagglutinins.
作者信息
HENLE G, GIRARDI A, HENLE W
出版信息
J Exp Med. 1955 Jan 1;101(1):25-41. doi: 10.1084/jem.101.1.25.
Abstract
Various strains of influenza virus produce a cytopathogenic effect in cultures of HeLa cells. The virus could not be passed in series. Virus partially or even completely inactivated with respect to infectivity by exposure to 37 degrees C. or ultraviolet light retained some of its cytopathogenic effect. No evidence has been obtained of an increase in infectious virus in HeLa cultures, but an increase in hemagglutinins and in both viral and soluble complement-fixing antigens became detectable during incubation. These virus materials apparently were not released from these cells prior to their destruction. These results suggested that HeLa cells are capable of supporting an incomplete reproductive cycle of influenza virus. The fact that radioactive phosphorus was readily incorporated into the hemagglutinin supplies strong evidence for this interpretation.
摘要
多种流感病毒株可在HeLa细胞培养物中产生细胞病变效应。该病毒无法连续传代。通过暴露于37摄氏度或紫外线，病毒的感染性部分甚至完全丧失，但仍保留了一些细胞病变效应。在HeLa细胞培养物中未发现感染性病毒增加的证据，但在培养期间可检测到血凝素以及病毒和可溶性补体结合抗原均有所增加。这些病毒物质显然在细胞被破坏之前并未从这些细胞中释放出来。这些结果表明，HeLa细胞能够支持流感病毒的不完全繁殖周期。放射性磷很容易掺入血凝素这一事实为这一解释提供了有力证据。
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