MAFFLY R H, LEAF A
J Gen Physiol. 1959 Jul 20;42(6):1257-75. doi: 10.1085/jgp.42.6.1257.
Melting point depression was used as an index of the water potential of rat tissues and serum. Organs removed from anesthetized rats were immediately frozen in liquid nitrogen and ground with mortar and pestle. Aliquots of the resulting frozen powder were suspended in chilled liquid silicone. While the suspension was vigorously stirred and warmed at a constant rate, the temperature of the melting mixture was measured. The melting curves of rat muscle, liver, heart, and brain were not significantly different from those of rat serum. The melting curve depression of whole kidney was greater than that of serum; this was demonstrated to be due to hypertonicity of the renal medullary area alone. It was demonstrated that autolysis will rapidly increase the depression of the melting curve of tissue. It is concluded that within the limits of the method used the melting point depression, and hence the water potential, of intracellular and extracellular fluids is the same.
熔点降低被用作大鼠组织和血清水势的指标。从麻醉大鼠身上取出的器官立即在液氮中冷冻，并用研钵和研杵研磨。将所得冷冻粉末的等分试样悬浮在冷却的液体硅酮中。在剧烈搅拌悬浮液并以恒定速率加热的同时，测量熔融混合物的温度。大鼠肌肉、肝脏、心脏和大脑的熔化曲线与大鼠血清的熔化曲线没有显著差异。全肾的熔化曲线降低大于血清；这被证明仅是由于肾髓质区域的高渗性。结果表明，自溶会迅速增加组织熔化曲线的降低。得出的结论是，在所使用方法的范围内，细胞内液和细胞外液的熔点降低以及因此的水势是相同的。
