PORTERFIELD J S
Bull World Health Organ. 1961;24(6):735-41.
In an extension of recent work on the antigenic interrelationships of arthropod-borne viruses, the plaque-inhibition test has been applied to the study of 15 Group A strains. Middelburg and eastern equine encephalomyelitis viruses show no relationship to any other virus in the group. Sindbis and western equine encephalomyelitis viruses show a one-way relationship only. The remaining viruses all share some antigenic components which react with hyperimmune rabbit sera prepared against Semliki Forest virus. However, using single-dose rabbit sera, or more specific mouse-immune sera, four distinct subgroups can be defined. One includes Semliki Forest virus strains; another Chikungunya virus and its substrains, Vereeniging and TH 35 viruses; the third contains O'nyong-nyong virus; and the fourth Mayaro and Uruma viruses.This paper also demonstrates how the plaque-inhibition technique may be used for the rapid identification of new virus isolates.
在近期关于虫媒病毒抗原相互关系研究的拓展中，蚀斑抑制试验已应用于对15株A组病毒的研究。米德尔堡病毒和东部马脑炎病毒与该组中的任何其他病毒均无关系。辛德毕斯病毒和西部马脑炎病毒仅呈现单向关系。其余病毒均共享一些抗原成分，这些成分可与针对塞姆利基森林病毒制备的超免疫兔血清发生反应。然而，使用单剂量兔血清或更具特异性的小鼠免疫血清，可以定义出四个不同的亚组。一个亚组包括塞姆利基森林病毒株；另一个包括基孔肯雅病毒及其亚株、韦伦埃宁病毒和TH 35病毒；第三个亚组包含奥尼昂尼昂病毒；第四个亚组包含马亚罗病毒和乌鲁马病毒。本文还展示了蚀斑抑制技术可如何用于新病毒分离株的快速鉴定。
