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伯氏疏螺旋体的93千道尔顿蛋白：一种免疫显性原生质圆柱体抗原。

The 93-kilodalton protein of Borrelia burgdorferi: an immunodominant protoplasmic cylinder antigen.

作者信息

Luft B J, Mudri S, Jiang W, Dattwyler R J, Gorevic P D, Fischer T, Munoz P, Dunn J J, Schubach W H

机构信息

Department of Medicine, State University of New York, Stony Brook 11794-8153.

出版信息

Infect Immun. 1992 Oct;60(10):4309-21. doi: 10.1128/iai.60.10.4309-4321.1992.

DOI:10.1128/iai.60.10.4309-4321.1992
PMID:1398941
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC257467/
Abstract

Using immunoblots, we identified proteins of Borrelia burgdorferi recognized by sera from 62 patients with either acute or chronic Lyme disease. In all groups studied, the 41-kDa flagellar protein and a relatively minor 93-kDa protein (p93) were the most commonly recognized antigens in patients with acute and chronic disease due to B. burgdorferi. A murine monoclonal antibody (MAb 181.1) was developed against p93, and the antigen was detected by immunoblot analysis in four European and American strains of B. burgdorferi. On two-dimensional gel electrophoresis, p93 had an apparent pI of 6.8. Immunoelectronmicroscopy with MAb 181.1 demonstrated that p93 is located within the protoplasmic cylinder compartment of the organism. The gene encoding p93 was retrieved from a phage expression library. The derived amino acid sequence of p93 confirmed chemical characterization of the antigen, including its amino-terminal peptide sequence. The derived amino acid sequence predicted it to be predominantly alpha helical. A prominent antigenic domain located at the carboxy portion of the protein was recognized by human and rabbit polyclonal antisera and human (MAb D4) and mouse (MAb 181.1) MAbs.

摘要

通过免疫印迹法，我们鉴定了62例急性或慢性莱姆病患者血清所识别的伯氏疏螺旋体蛋白。在所有研究组中，41 kDa鞭毛蛋白和相对次要的93 kDa蛋白（p93）是伯氏疏螺旋体所致急性和慢性疾病患者中最常被识别的抗原。针对p93制备了一种鼠单克隆抗体（MAb 181.1），并通过免疫印迹分析在4株欧美伯氏疏螺旋体菌株中检测到了该抗原。在二维凝胶电泳中，p93的表观等电点为6.8。用MAb 181.1进行免疫电子显微镜观察表明，p93位于该生物体的原生质圆柱体区内。从噬菌体表达文库中获得了编码p93的基因。推导的p93氨基酸序列证实了该抗原的化学特性，包括其氨基末端肽序列。推导的氨基酸序列预测它主要为α螺旋结构。位于该蛋白羧基部分的一个突出抗原结构域可被人及兔多克隆抗血清以及人源（MAb D4）和鼠源（MAb 181.1）单克隆抗体识别。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe4/257467/1d2fbe1d9e75/iai00034-0380-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe4/257467/7188bf69121d/iai00034-0375-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe4/257467/7976bd122cd1/iai00034-0375-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe4/257467/67d90f8d3c5c/iai00034-0376-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe4/257467/23baf8ac9fa1/iai00034-0377-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe4/257467/e5c3134dac6f/iai00034-0377-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe4/257467/a71a7c66a3c2/iai00034-0378-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe4/257467/d75cdae44597/iai00034-0379-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe4/257467/798b47407165/iai00034-0380-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe4/257467/1d2fbe1d9e75/iai00034-0380-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe4/257467/7188bf69121d/iai00034-0375-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe4/257467/7976bd122cd1/iai00034-0375-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe4/257467/67d90f8d3c5c/iai00034-0376-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe4/257467/23baf8ac9fa1/iai00034-0377-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe4/257467/e5c3134dac6f/iai00034-0377-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe4/257467/a71a7c66a3c2/iai00034-0378-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe4/257467/d75cdae44597/iai00034-0379-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe4/257467/798b47407165/iai00034-0380-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe4/257467/1d2fbe1d9e75/iai00034-0380-b.jpg

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本文引用的文献

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Lyme disease-a tick-borne spirochetosis?莱姆病——一种蜱传播的螺旋体病？
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Spirochetes isolated from the blood of two patients with Lyme disease.从两名莱姆病患者血液中分离出的螺旋体。
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Serologic diagnosis of Lyme borreliosis by using enzyme-linked immunosorbent assays with recombinant antigens.使用重组抗原酶联免疫吸附试验进行莱姆病螺旋体病的血清学诊断。
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The immunoglobulin (IgG) antibody response to OspA and OspB correlates with severe and prolonged Lyme arthritis and the IgG response to P35 correlates with mild and brief arthritis.针对OspA和OspB的免疫球蛋白（IgG）抗体反应与严重且持续时间长的莱姆关节炎相关，而针对P35的IgG反应与轻度且短暂的关节炎相关。
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Lyme disease-causing Borrelia species encode multiple lipoproteins homologous to peptide-binding proteins of ABC-type transporters.导致莱姆病的疏螺旋体物种编码多种脂蛋白，这些脂蛋白与ABC型转运蛋白的肽结合蛋白同源。
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