Shimizu N, Takeuchi Y, Naruse T, Inagaki M, Moriyama E, Gojobori T, Hoshino H
Department of Hygiene, Gunma University School of Medicine, Japan.
J Mol Evol. 1992 Oct;35(4):329-36. doi: 10.1007/BF00161170.
Five strains of human immunodeficiency virus type 1 (HIV-1) were isolated from five Japanese hemophilia patients. Two isolates, HIV-1[GUN-1] and HIV-1[GUN-2], were from brother patients with hemophilia B and the other three isolates, HIV-1[GUN-3], HIV-1[GUN-4], and HIV-1[GUN-5], were from hemophilia A patients. Another HIV-1 strain, HIV-1[GUN-6], was isolated from a Canadian male homosexual with AIDS. The restriction endonuclease cleavage maps of the proviral genomes of these six HIV-1 strains revealed that they were apparently different from each other. The phylogenetic trees constructed using restriction maps and nucleotide sequences were quite similar, indicating that phylogenetic analyses of Japanese HIV-1 isolates can be done using restriction maps of the proviruses. Phylogenetic analyses showed that they were more closely related to HIV-1s which had been reported to be isolated from homosexual patients in the United States than those isolated from African patients. In particular, GUN-1 and GUN-2 isolates were on the branch of a San Francisco isolate, ARV2, while GUN-5 and GUN-6 isolates were on the branch of HTLV-IIIB-related isolates.
从五名日本血友病患者中分离出了五株1型人类免疫缺陷病毒（HIV-1）。其中两株，HIV-1[GUN-1]和HIV-1[GUN-2]，来自患B型血友病的兄弟患者，另外三株，HIV-1[GUN-3]、HIV-1[GUN-4]和HIV-1[GUN-5]，来自A型血友病患者。另一株HIV-1毒株，HIV-1[GUN-6]，是从一名患有艾滋病的加拿大男性同性恋者身上分离出来的。这六株HIV-1毒株前病毒基因组的限制性内切酶切割图谱显示，它们彼此明显不同。利用限制性图谱和核苷酸序列构建的系统发育树非常相似，这表明可以使用前病毒的限制性图谱对日本HIV-1分离株进行系统发育分析。系统发育分析表明，与从非洲患者中分离出的HIV-1相比，它们与据报道从美国同性恋患者中分离出的HIV-1关系更为密切。特别是，GUN-1和GUN-2分离株位于旧金山分离株ARV2的分支上，而GUN-5和GUN-6分离株位于与HTLV-IIIB相关分离株的分支上。
