• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

原癌基因met和肝细胞生长因子基因的致瘤性。

Tumorigenicity of the met proto-oncogene and the gene for hepatocyte growth factor.

作者信息

Rong S, Bodescot M, Blair D, Dunn J, Nakamura T, Mizuno K, Park M, Chan A, Aaronson S, Vande Woude G F

机构信息

ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Maryland 21702.

出版信息

Mol Cell Biol. 1992 Nov;12(11):5152-8. doi: 10.1128/mcb.12.11.5152-5158.1992.

DOI:10.1128/mcb.12.11.5152-5158.1992
PMID:1406687
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC360449/
Abstract

The met proto-oncogene is the tyrosine kinase growth factor receptor for hepatocyte growth factor/scatter factor (HGF/SF). It was previously shown that, like the oncogenic tpr-met, the mouse met proto-oncogene transforms NIH 3T3 cells. We have established NIH 3T3 cells stably expressing both human (Methu) and mouse (Metmu) met proto-oncogene products. The protein products are properly processed and appear on the cell surface. NIH 3T3 cells express endogenous mouse HGF/SF mRNA, suggesting an autocrine activation mechanism for transformation by Metmu. However, the tumor-forming activity of Methu in NIH 3T3 cells is very low compared with that of Metmu, but efficient tumorigenesis occurs when Methu and HGF/SFhu are coexpressed. These results are consistent with an autocrine transformation mechanism and suggest further that the endogenous murine factor inefficiently activates the tumorigenic potential of Methu. The tumorigenicity observed with reciprocal chimeric human and mouse receptors that exchange external ligand-binding domains supports this conclusion. We also show that HGF/SFhu expressed in NIH 3T3 cells produces tumors in nude mice.

摘要

原癌基因met是肝细胞生长因子/分散因子（HGF/SF）的酪氨酸激酶生长因子受体。先前的研究表明，与致癌性tpr-met一样，小鼠原癌基因met可转化NIH 3T3细胞。我们已经建立了稳定表达人（Methu）和小鼠（Metmu）原癌基因met产物的NIH 3T3细胞系。蛋白质产物经过适当加工并出现在细胞表面。NIH 3T3细胞表达内源性小鼠HGF/SF mRNA，提示Metmu介导的转化存在自分泌激活机制。然而，与Metmu相比，Methu在NIH 3T3细胞中的致瘤活性非常低，但当Methu和HGF/SFhu共表达时，可有效发生肿瘤形成。这些结果与自分泌转化机制一致，并进一步表明内源性鼠源因子不能有效激活Methu的致瘤潜能。交换外部配体结合域的人源和鼠源相互嵌合受体所观察到的致瘤性支持了这一结论。我们还表明，在NIH 3T3细胞中表达的HGF/SFhu可在裸鼠中产生肿瘤。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75ca/360449/2d462d2d944f/molcellb00134-0358-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75ca/360449/89ba97f2c391/molcellb00134-0356-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75ca/360449/d67719750186/molcellb00134-0357-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75ca/360449/3b923a16d5c6/molcellb00134-0357-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75ca/360449/2d462d2d944f/molcellb00134-0358-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75ca/360449/89ba97f2c391/molcellb00134-0356-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75ca/360449/d67719750186/molcellb00134-0357-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75ca/360449/3b923a16d5c6/molcellb00134-0357-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75ca/360449/2d462d2d944f/molcellb00134-0358-a.jpg

相似文献

1
Tumorigenicity of the met proto-oncogene and the gene for hepatocyte growth factor.原癌基因met和肝细胞生长因子基因的致瘤性。
Mol Cell Biol. 1992 Nov;12(11):5152-8. doi: 10.1128/mcb.12.11.5152-5158.1992.
2
Tumorigenesis induced by coexpression of human hepatocyte growth factor and the human met protooncogene leads to high levels of expression of the ligand and receptor.人肝细胞生长因子与人类原癌基因met共表达诱导的肿瘤发生导致配体和受体的高表达。
Cell Growth Differ. 1993 Jul;4(7):563-9.
3
Invasiveness and metastasis of NIH 3T3 cells induced by Met-hepatocyte growth factor/scatter factor autocrine stimulation.Met-肝细胞生长因子/分散因子自分泌刺激诱导NIH 3T3细胞的侵袭和转移。
Proc Natl Acad Sci U S A. 1994 May 24;91(11):4731-5. doi: 10.1073/pnas.91.11.4731.
4
Expression of the proto-oncogenes c-met and c-kit and their ligands, hepatocyte growth factor/scatter factor and stem cell factor, in SCLC cell lines and xenografts.原癌基因c-met和c-kit及其配体肝细胞生长因子/分散因子和干细胞因子在小细胞肺癌细胞系及异种移植瘤中的表达。
Br J Cancer. 1993 Jan;67(1):37-46. doi: 10.1038/bjc.1993.7.
5
Autocrine mechanism for met proto-oncogene tumorigenicity.原癌基因met致瘤性的自分泌机制。
Cold Spring Harb Symp Quant Biol. 1994;59:629-36. doi: 10.1101/sqb.1994.059.01.072.
6
The Met proto-oncogene mesenchymal to epithelial cell conversion.原癌基因Met介导的间充质向上皮细胞转化。
Science. 1994 Jan 7;263(5143):98-101. doi: 10.1126/science.7505952.
7
The Met-HGF/SF autocrine signaling mechanism is involved in sarcomagenesis.Met-HGF/SF自分泌信号传导机制参与肉瘤发生。
EXS. 1995;74:89-121. doi: 10.1007/978-3-0348-9070-0_6.
8
Deletion of the ectodomain unleashes the transforming, invasive, and tumorigenic potential of the MET oncogene.胞外结构域的缺失释放了MET癌基因的转化、侵袭和致瘤潜能。
Cancer Sci. 2009 Apr;100(4):633-8. doi: 10.1111/j.1349-7006.2008.01079.x. Epub 2009 Jan 21.
9
Properties and functions of scatter factor/hepatocyte growth factor and its receptor c-Met.散射因子/肝细胞生长因子及其受体c-Met的特性与功能
Am J Respir Cell Mol Biol. 1993 Mar;8(3):229-37. doi: 10.1165/ajrcmb/8.3.229.
10
Efficient cell transformation by the Tpr-Met oncoprotein is dependent upon tyrosine 489 in the carboxy-terminus.Tpr-Met癌蛋白介导的高效细胞转化依赖于羧基末端的酪氨酸489。
Oncogene. 1995 Jan 19;10(2):237-49.

引用本文的文献

1
Dissecting metastasis using preclinical models and methods.使用临床前模型和方法解析转移。
Nat Rev Cancer. 2023 Jun;23(6):391-407. doi: 10.1038/s41568-023-00568-4. Epub 2023 May 3.
2
Application status and future prospects of the PDX model in lung cancer.PDX模型在肺癌中的应用现状与未来前景
Front Oncol. 2023 Mar 24;13:1098581. doi: 10.3389/fonc.2023.1098581. eCollection 2023.
3
MET exon 14 skipping mutation is a hepatocyte growth factor (HGF)-dependent oncogenic driver in vitro and in humanised HGF knock-in mice.

本文引用的文献

1
Molecular cloning of a new transforming gene from a chemically transformed human cell line.从化学转化的人细胞系中克隆一个新的转化基因
Nature. 1984;311(5981):29-33. doi: 10.1038/311029a0.
2
Partial purification and characterization of hepatocyte growth factor from serum of hepatectomized rats.从肝切除大鼠血清中部分纯化和鉴定肝细胞生长因子
Biochem Biophys Res Commun. 1984 Aug 16;122(3):1450-9. doi: 10.1016/0006-291x(84)91253-1.
3
New method for detecting cellular transforming genes.检测细胞转化基因的新方法。
MET 外显子 14 跳跃突变是体外和人源化 HGF 敲入小鼠中肝细胞生长因子 (HGF) 依赖性致癌驱动因素。
Mol Oncol. 2023 Nov;17(11):2257-2274. doi: 10.1002/1878-0261.13397. Epub 2023 Jul 14.
4
Functional signaling test identifies HER2 negative breast cancer patients who may benefit from c-Met and pan-HER combination therapy.功能信号检测可识别出可能从 c-Met 和泛 HER 联合治疗中获益的 HER2 阴性乳腺癌患者。
Cell Commun Signal. 2022 Jan 8;20(1):4. doi: 10.1186/s12964-021-00798-9.
5
The FAM3C locus that encodes interleukin-like EMT inducer (ILEI) is frequently co-amplified in MET-amplified cancers and contributes to invasiveness.FAM3C 基因座编码白细胞介素样 EMT 诱导因子（ILEI），在 MET 扩增的癌症中经常共同扩增，并有助于侵袭性。
J Exp Clin Cancer Res. 2021 Feb 17;40(1):69. doi: 10.1186/s13046-021-01862-5.
6
A receptor-antibody hybrid hampering MET-driven metastatic spread.一种受体-抗体杂交阻碍 MET 驱动的转移扩散。
J Exp Clin Cancer Res. 2021 Jan 14;40(1):32. doi: 10.1186/s13046-020-01822-5.
7
MET Inhibitors in Small Cell Lung Cancer: From the Bench to the Bedside.小细胞肺癌中的MET抑制剂：从实验室到临床
Cancers (Basel). 2019 Sep 20;11(10):1404. doi: 10.3390/cancers11101404.
8
MM-131, a bispecific anti-Met/EpCAM mAb, inhibits HGF-dependent and HGF-independent Met signaling through concurrent binding to EpCAM.MM-131，一种双特异性抗-Met/EpCAM mAb，通过同时结合 EpCAM，抑制 HGF 依赖性和非依赖性 Met 信号传导。
Proc Natl Acad Sci U S A. 2019 Apr 9;116(15):7533-7542. doi: 10.1073/pnas.1819085116. Epub 2019 Mar 21.
9
Failures in preclinical and clinical trials of c-Met inhibitors: evaluation of pathway activity as a promising selection criterion.c-Met抑制剂的临床前和临床试验失败：评估通路活性作为一种有前景的选择标准。
Oncotarget. 2019 Jan 4;10(2):184-197. doi: 10.18632/oncotarget.26546.
10
Activated HGF-c-Met Axis in Head and Neck Cancer.头颈部癌中激活的HGF-c-Met轴
Cancers (Basel). 2017 Dec 12;9(12):169. doi: 10.3390/cancers9120169.
Science. 1982 Dec 10;218(4577):1122-5. doi: 10.1126/science.6293052.
4
Autocrine growth factors and cancer.自分泌生长因子与癌症。
Nature. 1985;313(6005):745-7. doi: 10.1038/313745a0.
5
An epithelial scatter factor released by embryo fibroblasts.胚胎成纤维细胞释放的一种上皮散射因子。
J Cell Sci. 1985 Aug;77:209-23. doi: 10.1242/jcs.77.1.209.
6
Post-translational acquisition of insulin binding activity by the insulin proreceptor. Correlation to recognition by autoimmune antibody.胰岛素原受体通过翻译后修饰获得胰岛素结合活性。与自身免疫抗体识别的相关性。
J Biol Chem. 1987 May 15;262(14):6816-22.
7
Overexpression of the human EGF receptor confers an EGF-dependent transformed phenotype to NIH 3T3 cells.人表皮生长因子受体的过表达赋予NIH 3T3细胞一种依赖表皮生长因子的转化表型。
Cell. 1987 Dec 24;51(6):1063-70. doi: 10.1016/0092-8674(87)90592-7.
8
Epidermal-growth-factor-dependent transformation by a human EGF receptor proto-oncogene.人表皮生长因子受体原癌基因介导的表皮生长因子依赖性转化
Science. 1987 Dec 4;238(4832):1408-10. doi: 10.1126/science.3500513.
9
Frequent generation of oncogenes by in vitro recombination of TRK protooncogene sequences.
Proc Natl Acad Sci U S A. 1988 May;85(9):2964-8. doi: 10.1073/pnas.85.9.2964.
10
Purification and subunit structure of hepatocyte growth factor from rat platelets.大鼠血小板中肝细胞生长因子的纯化及亚基结构
FEBS Lett. 1987 Nov 30;224(2):311-6. doi: 10.1016/0014-5793(87)80475-1.