Jarry Hubertus, Spengler Barbara, Porzel Andrea, Schmidt Juergen, Wuttke Wolfgang, Christoffel Volker
Klinische und Experimentelle Endokrinologie, Universitätsfrauenklinik Göttingen, Göttingen, Germany.
Planta Med. 2003 Oct;69(10):945-7. doi: 10.1055/s-2003-45105.
Recent cell culture experiments indicated that extracts of Vitex agnus-castus (VAC) may contain yet unidentified phytoestrogens. Estrogenic actions are mediated via estrogen receptors (ER). To investigate whether VAC compounds bind to the currently known isoforms ERalpha or ERss, ligand binding assays (LBA) were performed. Subtype specific ER-LBA revealed a binding of VAC to ERss only. To isolate the ERss-selective compounds, the extract was fractionated by bio-guidance. The flavonoid apigenin was isolated and identified as the most active ERss-selective phytoestrogen in VAC. Other isolated compounds were vitexin and penduletin. These data demonstrate that the phytoestrogens in VAC are ERss-selective.
近期的细胞培养实验表明，贞洁树（VAC）提取物可能含有尚未明确的植物雌激素。雌激素作用是通过雌激素受体（ER）介导的。为了研究VAC化合物是否与目前已知的ERα或ERβ亚型结合，进行了配体结合试验（LBA）。亚型特异性ER-LBA显示VAC仅与ERβ结合。为了分离出ERβ选择性化合物，通过生物导向对提取物进行了分级分离。黄酮类芹菜素被分离出来，并被鉴定为VAC中最具活性的ERβ选择性植物雌激素。其他分离出的化合物是牡荆素和藤黄菌素。这些数据表明，VAC中的植物雌激素具有ERβ选择性。
