钙离子参与大肠杆菌的趋化作用。
Calcium ions are involved in Escherichia coli chemotaxis.
作者信息
Tisa L S, Adler J
机构信息
Department of Biochemistry, University of Wisconsin, Madison 53706.
出版信息
Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11804-8. doi: 10.1073/pnas.89.24.11804.
Abstract
Escherichia coli regulates intracellular free Ca2+ at about 90 nM [Gangola, P. & Rosen, B. P. (1987) J. Biol. Chem. 262, 12570-12574]. To increase intracellular free Ca2+, nitr-5/Ca2+, a "caged" Ca2+ compound, was electroporated into cells and then its affinity for Ca2+ was reduced by exposure to 370-nm light. Upon release of the Ca2+ ions, the cells tumbled. Studies on mutant strains showed that the receptor proteins (methyl-accepting chemotaxis proteins, MCPs) were not required for the Ca(2+)-induced tumbling but that CheA, CheW, and CheY proteins were required. Similar results were obtained with DM-nitrophen/Ca2+, another caged calcium compound that releases Ca2+ upon illumination at 340 nm. Diazo-2, a caged Ca2+ chelator that takes up Ca2+ upon illumination at 340 nm, was used to decrease intracellular free Ca2+, and this caused smooth swimming.
摘要
大肠杆菌将细胞内游离钙离子浓度调节在约90纳摩尔[甘戈拉，P. & 罗森，B. P.（1987年）《生物化学杂志》262卷，第12570 - 12574页]。为了增加细胞内游离钙离子浓度，“笼状”钙离子化合物硝普钠 - 5/钙离子通过电穿孔导入细胞，然后通过暴露于370纳米波长的光来降低其对钙离子的亲和力。钙离子释放后，细胞发生翻滚。对突变菌株的研究表明，钙离子诱导的翻滚不需要受体蛋白（甲基化趋化蛋白，MCPs），但需要CheA、CheW和CheY蛋白。用另一种笼状钙化合物二甲基硝基苯酚/钙离子（在340纳米光照下释放钙离子）也得到了类似结果。重氮 - 2是一种笼状钙离子螯合剂，在340纳米光照下摄取钙离子，用于降低细胞内游离钙离子浓度，这会导致细胞平稳游动。
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本文引用的文献
1
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J Bacteriol. 1981 Mar;145(3):1209-21. doi: 10.1128/jb.145.3.1209-1221.1981.
2
Change in intracellular pH of Escherichia coli mediates the chemotactic response to certain attractants and repellents.大肠杆菌细胞内pH值的变化介导了对某些引诱剂和驱避剂的趋化反应。
J Bacteriol. 1981 Mar;145(3):1196-208. doi: 10.1128/jb.145.3.1196-1208.1981.
3
Role of membrane potential and calcium in chemotactic sensing by bacteria.膜电位和钙在细菌趋化感应中的作用。
J Mol Biol. 1981 Jun 25;149(2):241-57. doi: 10.1016/0022-2836(81)90300-4.
4
Negative chemotaxis in Escherichia coli.大肠杆菌中的负趋化性。
J Bacteriol. 1974 May;118(2):560-76. doi: 10.1128/jb.118.2.560-576.1974.
5
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J Bacteriol. 1972 Oct;112(1):315-26. doi: 10.1128/jb.112.1.315-326.1972.
6
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J Bacteriol. 1987 May;169(5):1878-85. doi: 10.1128/jb.169.5.1878-1885.1987.
7
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8
Sensory transduction in bacterial chemotaxis involves phosphotransfer between Che proteins.细菌趋化作用中的感觉转导涉及Che蛋白之间的磷酸转移。
Biochem Biophys Res Commun. 1988 Mar 15;151(2):891-6. doi: 10.1016/s0006-291x(88)80365-6.
9
Calcium channel blockers inhibit bacterial chemotaxis.钙通道阻滞剂抑制细菌趋化性。
FEBS Lett. 1988 Aug 29;236(2):437-40. doi: 10.1016/0014-5793(88)80072-3.
10
Mutations specifically affecting ligand interaction of the Trg chemosensory transducer.特异性影响Trg化学感应转导器配体相互作用的突变。
J Bacteriol. 1986 Jul;167(1):101-9. doi: 10.1128/jb.167.1.101-109.1986.
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