Pan Y T, Xie T Q, Du C W, Bastacky S, Meyers S, Zeidel M L
Department of Biomedical Engineering, State University of New York at Stony Brook, Stony Brook, New York 11794-8181, USA.
Opt Lett. 2003 Dec 15;28(24):2485-7. doi: 10.1364/ol.28.002485.
We report an experimental study of the possibility of enhancing early bladder cancer diagnosis with fluorescence-image-guided endoscopic optical coherence tomography (OCT). After the intravesical instillation of a 10% solution of 5-aminolevulinic acid, simultaneous fluorescence imaging (excitation of 380-420 nm, emission of 620-700 nm) and OCT are performed on rat bladders to identify the photochemical and morphological changes associated with uroepithelial tumorigenesis. The preliminary results of our ex vivo study reveal that both fluorescence and OCT can identify early uroepithelial cancers, and OCT can detect precancerous lesions (e.g., hyperplasia) that fluorescence may miss. This suggests that a cystoscope combining 5-aminolevulinic acid fluorescence and OCT imaging has the potential to enhance the efficiency and sensitivity of early bladder cancer diagnosis.
我们报告了一项关于利用荧光图像引导的内镜光学相干断层扫描（OCT）增强早期膀胱癌诊断可能性的实验研究。在膀胱内灌注10%的5-氨基乙酰丙酸溶液后，对大鼠膀胱同时进行荧光成像（激发波长380 - 420nm，发射波长620 - 700nm）和OCT检查，以识别与尿路上皮肿瘤发生相关的光化学和形态学变化。我们体外研究的初步结果表明，荧光和OCT都能识别早期尿路上皮癌，并且OCT能够检测出荧光可能遗漏的癌前病变（如增生）。这表明，结合5-氨基乙酰丙酸荧光和OCT成像的膀胱镜有潜力提高早期膀胱癌诊断的效率和灵敏度。
