Kotliar Konstantin E, Vilser Walthard, Nagel Edgar, Lanzl Ines M
Augenklinik rechts der Isar, Technical University of Munich, Ismaninger Str. 22, 81675, Germany.
Graefes Arch Clin Exp Ophthalmol. 2004 May;242(5):377-92. doi: 10.1007/s00417-003-0847-x. Epub 2004 Feb 10.
Flickering light stimulation of the retina is known to increase retinal vessel diameter in animals and humans. The aim of the study was to quantify the response of retinal vessel diameter to red-green and blue-green flickering light.
In 11 normal healthy volunteers (mean age: 25.2+/-6.8 years) retinal arterial and venous diameters were examined by Retinal Vessel Analyzer (IMEDOS Ltd., Weimar, Germany) before, during and after red-green and blue-green flicker stimulation with a frequency of 12 Hz and duration of 10 and 30 s.
For red-green flicker at 10 s there was a 2.4+/-1.4% arterial diameter increase at 9.1+/-3.3 s with a return to baseline after 30 s and a 2.4+/-1.1% venous diameter increase at 12.1+/-2.6 s with a return to baseline after 30 s. For red-green flicker at 30 s there was a 3.2+/-1.5% arterial diameter increase at 26.9+/-12.6 s with a return to baseline after 40 s and a 4.9+/-1.8% venous diameter increase at 31.4+/-7.6 s with a return to baseline after 40 s. For blue-green flicker at 10 s there was a 2.0+/-0.7% arterial diameter increase at 10.6+/-5.3 s with a return to baseline after 30 s and a 2.3+/-1.1% venous diameter increase at 12.0+/-5.5 s with a return to baseline after 30 s. For blue-green flicker at 30 s there was a 2.6+/-1.3% arterial diameter increase at 20.7+/-8.0 s with a return to baseline after 40 s and a 3.4+/-2.2% venous diameter increase at 28.8+/-10.5 s with a return to baseline after 40 s.
Retinal vessel diameter dilation is a reproducible response to the applied flicker stimuli. This finding supports the existence of neurovascular coupling in the human retina. Flicker stimulation in either red-green or blue-green might be a useful stimulus for examination of retinal vessel behavior to regulatory demands.
已知视网膜的闪烁光刺激可增加动物和人类的视网膜血管直径。本研究的目的是量化视网膜血管直径对红 - 绿和蓝 - 绿闪烁光的反应。
在11名正常健康志愿者（平均年龄：25.2±6.8岁）中，使用视网膜血管分析仪（德国魏玛IMEDOS有限公司）在12Hz频率、持续10秒和30秒的红 - 绿及蓝 - 绿闪烁光刺激前、刺激期间和刺激后检查视网膜动脉和静脉直径。
对于10秒的红 - 绿闪烁，在9.1±3.3秒时动脉直径增加2.4±1.4%，30秒后恢复到基线；在12.1±2.6秒时静脉直径增加2.4±1.1%，30秒后恢复到基线。对于30秒 的红 - 绿闪烁,在26.9±12.6秒时动脉直径增加3.2±1.5%，40秒后恢复到基线；在31.4±7.6秒时静脉直径增加4.9±1.8%，40秒后恢复到基线。对于10秒的蓝 - 绿闪烁，在10.6±5.3秒时动脉直径增加2.0±0.7%，30秒后恢复到基线；在12.0±5.5秒时静脉直径增加2.3±1.1%，30秒后恢复到基线。对于30秒的蓝 - 绿闪烁，在20.7±8.0秒时动脉直径增加2.6±1.3%，40秒后恢复到基线；在28.8±10.5秒时静脉直径增加3.4±2.2%，40秒后恢复到基线。
视网膜血管直径扩张是对所施加闪烁刺激可重复的反应。这一发现支持了人类视网膜中神经血管耦合的存在。红 - 绿或蓝 - 绿的闪烁刺激可能是用于检查视网膜血管对调节需求反应的有用刺激。
