Takao Ayuko, Nagamune Hideaki, Maeda Nobuko
Department of Oral Bacteriology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan.
FEMS Microbiol Lett. 2004 Apr 1;233(1):83-9. doi: 10.1016/j.femsle.2004.01.042.
The aim of this study was to establish an identification method for the anginosus group within the genus Streptococcus by polymerase chain reaction (PCR). Using a primer pair based on the group-specific sequences of penicillin-binding protein 2B (pbp2b) gene, a 275-bp fragment was amplified from each species in the group but no size-matched products were obtained in other streptococci. Further identification in the species or subspecies level was possible by a multiplex PCR with primers for the 16S ribosomal RNA gene of Streptococcus anginosus, the hyaluronate lyase genes both of Streptococcus intermedius and Streptococcus constellatus subsp. constellatus, and the intermedilysin (ily) gene of S. intermedius. In the case ofStreptococcus constellatus subsp. pharyngis, the amplified fragment from the S. intermedius-type hyaluronate lyase gene was obtained, while that from the ily gene was not. These results also indicate that two different hyaluronate lyase genes are distributed among the anginosus group.
本研究的目的是通过聚合酶链反应（PCR）建立一种鉴定链球菌属中咽峡炎菌群的方法。使用基于青霉素结合蛋白2B（pbp2b）基因的组特异性序列的引物对，从该菌群的每个物种中扩增出一个275bp的片段，但在其他链球菌中未获得大小匹配的产物。通过使用针对咽峡炎链球菌16S核糖体RNA基因、中间链球菌和星座链球菌亚种星座亚种的透明质酸裂解酶基因以及中间链球菌的中间溶菌素（ily）基因的引物进行多重PCR，可以在物种或亚种水平上进行进一步鉴定。对于咽峡炎星座链球菌，获得了来自中间链球菌型透明质酸裂解酶基因的扩增片段，但未获得来自ily基因的扩增片段。这些结果还表明，两种不同的透明质酸裂解酶基因分布在咽峡炎菌群中。
