玻璃体内注射吲哚菁绿对兔的影响。

Effects of intravitreal indocyanine green injection in rabbits.

作者信息

Maia Mauricio, Margalit Eyal, Lakhanpal Rohit, Tso Mark O M, Grebe Rhonda, Torres Gustavo, Au Eong Kah-Guan, Farah Michel E, Fujii Gildo Y, Weiland James, de Juan Eugene, D'Anna Salvadori A, Humayun Mark S

机构信息

Wilmer Ophthalmological Institute, The Johns Hopkins University School of Medicine, 550 North Broadway, Baltimore, MD 21205, USA.

出版信息

Retina. 2004 Feb;24(1):69-79. doi: 10.1097/00006982-200402000-00011.

DOI:10.1097/00006982-200402000-00011

PMID:15076947

Abstract

PURPOSE

To report the clinical, electrophysiologic, and histologic findings of different concentrations of indocyanine green (ICG) injected into the vitreous cavity of rabbit eyes.

METHODS

Forty-two rabbits underwent intravitreal injection of 0.1 mL of ICG in three different concentrations: 0.5 mg/mL (250 mOsm), 5 mg/mL (270 mOsm), and 25 mg/mL (170 mOsm). Fellow eyes were injected with 0.1 mL of balanced salt solution. Biomicroscopy, ophthalmoscopy, electroretinography, fluorescein angiography, and histologic evaluation were performed.

RESULTS

Eyes injected with 0.5 mg/mL of ICG showed b-wave latency delay on the first day after injection. Eyes injected with 5 mg/mL of ICG showed b-wave latency delay and decreased b-wave amplitude on the first and seventh days after injection; delayed a-wave latency on the first day after injection was also observed. Eyes injected with 25 mg/mL of ICG showed b- and a-wave amplitude and latency abnormalities during the entire follow-up. Direct correlation of increasing retinal edema proportional to the progressively increasing ICG concentrations was shown on histologic evaluation.

CONCLUSION

Intravitreal ICG injection in rabbit eyes may impair retinal function and morphology proportional to the progressively increasing ICG dosages.

摘要

目的

报告向兔眼玻璃体内注射不同浓度吲哚菁绿（ICG）后的临床、电生理和组织学结果。

方法

42只兔子接受玻璃体内注射0.1 mL三种不同浓度的ICG：0.5 mg/mL（250 mOsm）、5 mg/mL（270 mOsm）和25 mg/mL（170 mOsm）。对侧眼注射0.1 mL平衡盐溶液。进行了生物显微镜检查、检眼镜检查、视网膜电图检查、荧光素血管造影检查和组织学评估。

结果

注射0.5 mg/mL ICG的眼睛在注射后第一天显示b波潜伏期延迟。注射5 mg/mL ICG的眼睛在注射后第一天和第七天显示b波潜伏期延迟和b波振幅降低；在注射后第一天还观察到a波潜伏期延迟。注射25 mg/mL ICG的眼睛在整个随访期间显示b波和a波振幅及潜伏期异常。组织学评估显示视网膜水肿程度与ICG浓度逐渐增加呈直接相关。