Di Stasi Luiz C, Camuesco Desireé, Nieto Ana, Vilegas Wagner, Zarzuelo Antonio, Galvez Julio
Department of Pharmacology, IB-UNESP, Botucatu, SP, Brazil.
Planta Med. 2004 Apr;70(4):315-20. doi: 10.1055/s-2004-818942.
The preventative intestinal anti-inflammatory activity of paepalantine, an isocoumarin isolated from the capitula of Paepalanthus bromelioides, was tested in the trinitrobenzenesulphonic acid (TNBS) model of rat colitis. This was performed in two different experimental settings, i. e. when the colonic mucosa is intact or when the mucosa is in process of recovery after an initial insult. The results obtained revealed that the paepalantine pretreatment, at doses of 5 and 10 mg/kg, significantly attenuated the colonic damage induced by TNBS in both situations, as it was evidenced both histologically and biochemically. This beneficial effect was associated with an improvement in the colonic oxidative status, since paepalantine prevented the glutathione depletion that occurred as a consequence of the colonic inflammation. In addition, the intestinal anti-inflammatory effect exerted by this isocoumarin was associated with an inhibition of colonic nitric oxide activity, which is upregulated as a consequence of the inflammatory process. In conclusion, the preventative effect exerted by paepalantine in the TNBS model of rat colitis is probably related with its antioxidant properties.
从凤梨叶拟灯台莲头状花序中分离得到的异香豆素——凤梨素的肠道抗炎预防活性，在大鼠结肠炎的三硝基苯磺酸（TNBS）模型中进行了测试。该实验在两种不同的实验条件下进行，即结肠黏膜完整时或黏膜在初次损伤后处于恢复过程中时。所获结果表明，在两种情况下，5和10 mg/kg剂量的凤梨素预处理均能显著减轻TNBS诱导的结肠损伤，这在组织学和生物化学上均得到了证实。这种有益作用与结肠氧化状态的改善相关，因为凤梨素可防止因结肠炎症而发生的谷胱甘肽耗竭。此外，这种异香豆素发挥的肠道抗炎作用与结肠一氧化氮活性的抑制相关，该活性因炎症过程而上调。总之，凤梨素在大鼠结肠炎TNBS模型中发挥的预防作用可能与其抗氧化特性有关。
