从自然种群中分离出的大肠杆菌菌株间的质粒转移率。
Rate of plasmid transfer among Escherichia coli strains isolated from natural populations.
作者信息
Gordon D M
机构信息
Department of Zoology, University of Massachusetts, Amherst 01003.
出版信息
J Gen Microbiol. 1992 Jan;138(1):17-21. doi: 10.1099/00221287-138-1-17.
The conjugative plasmid R1 was introduced into ten strains of Escherichia coli isolated from natural populations. Spontaneous nalidixic-acid-resistant mutants of the ten strains served as recipients. The ten donor and recipient strains were mated in all combinations and the rate at which R1 transferred between the strains was determined. The rate of transfer ranged from 5.2 x 10(-11)-1.1 x 10(-18) ml per cell h-1, and averaged 1.3 x 10(-15) ml per cell h-1. The results of these experiments suggest that the rates of conjugative transfer are far too low for plasmids to be maintained as parasites in their host populations. Infectious transfer is insufficient; plasmids must confer a selective advantage to their host to be maintained.
将接合质粒R1导入从自然群体中分离出的10株大肠杆菌。这10株菌株的自发耐萘啶酸突变体用作受体。将10个供体菌株和受体菌株进行所有组合的配对，并测定R1在菌株间的转移速率。转移速率范围为每细胞每小时5.2×10⁻¹¹ - 1.1×10⁻¹⁸毫升，平均为每细胞每小时1.3×10⁻¹⁵毫升。这些实验结果表明，接合转移速率过低，质粒无法作为寄生物在其宿主群体中维持。感染性转移不足；质粒必须赋予其宿主选择性优势才能得以维持。
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