Wiegand Annette, Krieger Christian, Attin Rengin, Hellwig Elmar, Attin Thomas
Department of Operative Dentistry, Preventive Dentistry and Periodontology, University of Göttingen, Robert-Koch-Str. 40, 37075, Göttingen, Germany.
Clin Oral Investig. 2005 Mar;9(1):52-7. doi: 10.1007/s00784-005-0306-7. Epub 2005 Feb 23.
The objective of this study was to analyse fluoride uptake and microhardness alteration of carious-like demineralised enamel after application of differently concentrated acidulated sodium fluoride gels and to determine the effect of fluoridation on enamel resistance against subsequent demineralisation. Artificial caries-like lesions of bovine enamel specimens were treated with sodium fluoride gels of different concentration (group A: 1.25%, group B: 0.62%, group C: 0.31%, group D: 0.15%; n=20 each group) for 5 min and stored in artificial saliva for 24 h. This cycle was carried out three times. Subsequently, KOH-soluble and structurally bound fluoride (determined at depths of 30, 60, and 90 microm) were analysed. In the second part of the study, for each 12 enamel specimens surface microhardness was determined before and after demineralisation, after fluoridation with the differently concentrated gels A-D, and after a second demineralisation. With all groups uptake of KOH-soluble and structurally bound fluoride resulted in higher levels than baseline content. Statistical analysis revealed significant differences between fluoride uptake among the groups, with highest uptake for the 1.25% gel and lowest for the 0.15% gel. Moreover, with all gels highest uptake was observed in the outermost enamel layer (P<0.05). Microhardness values after second demineralisation increased with increased concentration of the applied sodium fluoride gel. Increasing concentration of the applied gel implies better protection of the enamel specimens against subsequent demineralisation (P<0.05). It is concluded that differently concentrated acidulated sodium fluoride gels resulted in concentration-related significant uptake of fluoride in carious-like demineralised enamel, leading to a better demineralisation protection with increasing fluoride concentration in the gel.
本研究的目的是分析应用不同浓度的酸化氟化钠凝胶后，类龋脱矿釉质的氟摄取及显微硬度变化，并确定氟化处理对釉质抵抗后续脱矿的影响。将牛牙釉质标本的人工类龋损用不同浓度的氟化钠凝胶处理（A组：1.25%，B组：0.62%，C组：0.31%，D组：0.15%；每组n = 20）5分钟，然后在人工唾液中保存24小时。此循环进行3次。随后，分析KOH可溶性氟和结构结合氟（在30、60和90微米深度处测定）。在研究的第二部分，对12个釉质标本在脱矿前、用不同浓度的A - D组凝胶氟化后以及第二次脱矿后测定表面显微硬度。所有组的KOH可溶性氟和结构结合氟摄取量均高于基线含量。统计分析显示各组间氟摄取存在显著差异，1.25%凝胶的摄取量最高，0.15%凝胶的摄取量最低。此外，所有凝胶在最外层釉质中的摄取量最高（P<0.05）。第二次脱矿后的显微硬度值随所应用的氟化钠凝胶浓度增加而升高。所应用凝胶浓度的增加意味着对釉质标本抵抗后续脱矿的保护作用更好（P<0.05）。结论是，不同浓度的酸化氟化钠凝胶导致类龋脱矿釉质中氟摄取与浓度相关且具有显著差异，随着凝胶中氟浓度的增加，对脱矿的保护作用更好。
