基因工程葡萄酒酵母可产生高浓度、光学纯度极高的L-乳酸。
Genetically engineered wine yeast produces a high concentration of L-lactic acid of extremely high optical purity.
作者信息
Saitoh Satoshi, Ishida Nobuhiro, Onishi Toru, Tokuhiro Kenro, Nagamori Eiji, Kitamoto Katsuhiko, Takahashi Haruo
机构信息
Toyota Biotechnology & Afforestation Laboratory, Aichi 480-1192, Japan.
出版信息
Appl Environ Microbiol. 2005 May;71(5):2789-92. doi: 10.1128/AEM.71.5.2789-2792.2005.
Abstract
For mass production of lactic acid, we newly constructed a transgenic wine yeast strain that included six copies of the bovine L-lactate dehydrogenase gene on the genome. On fermentation in inexpensive cane juice-based medium, L-lactate production of this recombinant reached 122 g/liter and the optical purity was 99.9% or higher.
摘要
为了大规模生产乳酸，我们新构建了一种转基因葡萄酒酵母菌株，该菌株在基因组上包含六个拷贝的牛L-乳酸脱氢酶基因。在基于廉价甘蔗汁的培养基中发酵时，这种重组体的L-乳酸产量达到122克/升，光学纯度为99.9%或更高。
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