Farrant J, Knight S C
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3507-10. doi: 10.1073/pnas.76.7.3507.
The phytohemagglutinin-stimulated uptake of [3H]thymidine in mixtures of human lymphocytes from the same source was shown to depend on the cell concentration in vitro as well as on the period of cultivation. "Helper" and "suppressor" effects were obtained by varying the concentration of cells and the periods of cultivation. The possibility that helper and suppressor subpopulations were responsible was avoided by mixing lymphoid cell line cells with others of the same monoclonal origin. Even under these conditions, both the direction and the extent of activity depended on the same two variables. This weakens the case for postulating the existence of distinct subpopulations of lymphocytes with helper or suppressor properties. This case was based on the use of damaging treatments believed to separate cell populations which were then found to differ in their helper and suppressor properties. We propose instead that the effect of such treatments is mediated through changes in the concentrations of interacting cells. Our data make it clear that the function of lymphoid cells ascertained in one set of conditions need not apply within a different cellular environment.
结果表明，来自同一来源的人淋巴细胞混合物中，植物血凝素刺激的[3H]胸腺嘧啶核苷摄取量取决于体外细胞浓度以及培养时间。通过改变细胞浓度和培养时间获得了“辅助”和“抑制”效应。将淋巴母细胞系细胞与相同单克隆来源的其他细胞混合，避免了辅助和抑制亚群起作用的可能性。即使在这些条件下，活性的方向和程度仍取决于相同的两个变量。这削弱了假定存在具有辅助或抑制特性的不同淋巴细胞亚群的论据。这一论据基于使用了被认为可分离细胞群体的损伤性处理方法，随后发现这些细胞群体在辅助和抑制特性上存在差异。相反，我们认为此类处理的效应是通过相互作用细胞浓度的变化介导的。我们的数据清楚地表明，在一组条件下确定的淋巴样细胞功能在不同的细胞环境中不一定适用。
