Reisner Y, Pahwa S, Chiao J W, Sharon N, Evans R L, Good R A
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6778-82. doi: 10.1073/pnas.77.11.6778.
Soybean agglutinin (SBA) binds specifically to mouse B cells and has been used in the past to separate mouse B and T spleen cells by differential agglutination of the B cells. In the present study it was found that a major T-cell subpopulation of human peripheral blood mononuclear cells is agglutinated by SBA along with the B cells and monocytes. Tests of such cell surface markers as Fc receptors for IgG and IgM, as well as functional assays of antibody production by B cells, revealed that the SBA-agglutinated cell fraction contains the antibody helper T cells whereas the unagglutinated fraction is enriched with antibody suppressor T cells. Similar observations were made in tests of the proliferative response to mumps antigen. A recently prepared monoclonal antibody, anti-Leu 2a, which recognized the same thymus-dependent antigen previously defined by a heterologous anti-human T cell serum (alpha TH2), was found to define by indirect immunofluorescence a subpopulation of SBA- cells of intermediate staining intensity which was not detectable in the SBA+ population.
大豆凝集素（SBA）能特异性结合小鼠B细胞，过去曾用于通过对B细胞的差异凝集来分离小鼠B和T脾细胞。在本研究中发现，人外周血单个核细胞的一个主要T细胞亚群与B细胞和单核细胞一起被SBA凝集。对诸如IgG和IgM的Fc受体等细胞表面标志物的检测，以及对B细胞抗体产生的功能测定，显示SBA凝集的细胞部分含有抗体辅助性T细胞，而未凝集部分富含抗体抑制性T细胞。在对腮腺炎抗原的增殖反应测试中也有类似观察结果。一种最近制备的单克隆抗体，抗-Leu 2a，它识别先前由异源抗人T细胞血清（αTH2）定义的相同胸腺依赖性抗原，通过间接免疫荧光法发现它能定义出一群SBA-细胞，其染色强度中等，在SBA+群体中无法检测到。
