Park Soojin, Yeo Marie, Jin Ju-Hyun, Lee Ki-Myung, Jung Jae-Yeon, Choue Ryowon, Cho Sung Won, Hahm Ki-Baik
Genomic Research Center for Gastroenterology, Ajou University School of Medicine, Suwon, Kyungkido, Republic of Korea.
Dig Dis Sci. 2005 Jul;50(7):1218-27. doi: 10.1007/s10620-005-2763-x.
Helicobacter pylori has been known to provoke gastric inflammation, ulceration, and DNA damage, based on which WHO defined H. pylori as a class I carcinogen. Although ginseng, the root of Panax ginseng C.A. Meyer, has been reported to possess antiadhesion or antimicrobial activity against H. pylori, in this study, we examined the protective effect of red ginseng extracts (RGE) against H. pylori-induced cytotoxicity and DNA damage. RGE significantly attenuated both H. pylori-induced DNA damage assessed by comet assay and apoptosis measured by DNA fragmentation. Inactivation of ERK1/2 signaling and attenuation of caspase-3 activation and PARP cleavage were revealed with RGE against H. pylori infection. RGE decreased H. pylori-stimulated IL-8 gene expression, which resulted from the transcriptional regression of NF-kappaB. In conclusion, RGE showed significant gastroprotective effects against H. pylori-associated gastric mucosal cell damage, suggesting that red ginseng could be used as a medicinal phytonutrient against H. pylori infection.
幽门螺杆菌已知会引发胃部炎症、溃疡和DNA损伤，基于此，世界卫生组织将幽门螺杆菌定义为I类致癌物。虽然人参（五加科植物人参的根）已被报道具有抗幽门螺杆菌的黏附或抗菌活性，但在本研究中，我们检测了红参提取物（RGE）对幽门螺杆菌诱导的细胞毒性和DNA损伤的保护作用。RGE显著减轻了通过彗星试验评估的幽门螺杆菌诱导的DNA损伤以及通过DNA片段化检测的细胞凋亡。RGE可使ERK1/2信号失活，并减轻幽门螺杆菌感染诱导的caspase-3激活和PARP裂解。RGE降低了幽门螺杆菌刺激的IL-8基因表达，这是由NF-κB的转录下调导致的。总之，RGE对幽门螺杆菌相关的胃黏膜细胞损伤具有显著的胃保护作用，表明红参可作为一种对抗幽门螺杆菌感染的药用植物营养素。
