Cox M, Eveson J, Scully C
Centre for the Study of Oral Disease, University Department of Oral Medicine, Surgery and Pathology, Bristol Dental Hospital and School, England.
J Oral Pathol Med. 1991 Mar;20(3):143-5. doi: 10.1111/j.1600-0714.1991.tb00909.x.
DNA was extracted from an odontogenic keratocyst and assayed for the presence of human papilloma virus (HPV) type 16 DNA sequences using high stringency Southern blot hybridization. HPV type 16 homologous DNA sequences were detected at a copy number of 50-100 genome copies per diploid cell. The oral HPV DNA was not identical to the prototype HPV 16 when cleaved with the restriction enzyme Pst-I, since it appeared to lack the Pst-I C fragment (L2/L1 ORFs) and contained "off-sized" high molecular weight fragments suggestive of integration events into the host cell chromosome.
从牙源性角化囊肿中提取DNA，并使用高严谨度Southern印迹杂交法检测16型人乳头瘤病毒（HPV）DNA序列的存在情况。检测到16型HPV同源DNA序列，每个二倍体细胞中的拷贝数为50 - 100个基因组拷贝。当用限制性内切酶Pst - I切割时，口腔HPV DNA与原型HPV 16不同，因为它似乎缺少Pst - I C片段（L2/L1开放阅读框），并且包含提示整合到宿主细胞染色体中的“异常大小”的高分子量片段。
