Center for Disease Control, Atlanta, Georgia 30333.
Infect Immun. 1974 Nov;10(5):1040-3. doi: 10.1128/iai.10.5.1040-1043.1974.
Gonococcal infections of relatively long duration were produced in guinea pigs with minimal infective doses ranging from 10(1) to 10(2) colony-forming units. After spontaneous eradication and upon rechallenge with >/=10(8) gonococcal colony-forming units, guinea pigs were refractory to infection. Serum from these guinea pigs was bactericidal in vitro and protected virgin guinea pigs from in vivo challenge. The 7S peak, but not the 19S peak, from gel filtered immune serum demonstrated both bactericidal and passive protective properties. In vitro bactericidal activity of whole immune serum and its 7S peak was abolished after heating at 56 C for 30 min. The in vivo protective ability of the 7S peak was not abolished after heating.
用 10(1)到 10(2)个集落形成单位的最小感染剂量，在豚鼠中产生了持续时间相对较长的淋球菌感染。在自发清除后，用>/=10(8)个淋球菌集落形成单位再次攻击时，豚鼠对感染具有抗性。这些豚鼠的血清在体外具有杀菌作用，并能保护处女豚鼠免受体内攻击。从凝胶过滤免疫血清中得到的 7S 峰，但不是 19S 峰，具有杀菌和被动保护作用。整个免疫血清及其 7S 峰的体外杀菌活性在 56°C 加热 30 分钟后被消除。7S 峰的体内保护能力在加热后并未丧失。
