Quarta Angela, Mita Giovanni, Haidukowski Miriam, Logrieco Antonio, Mulè Giuseppina, Visconti Angelo
Institute of Sciences of Food Production, ISPA, National Research Council, CNR, Lecce, Italy.
FEMS Microbiol Lett. 2006 Jun;259(1):7-13. doi: 10.1111/j.1574-6968.2006.00235.x.
The ability to rapidly distinguish trichothecene chemotypes in a given species/population of the genus Fusarium is important due to significant differences in the toxicity of these secondary metabolites. A multiplex PCR assay, based on primer pairs derived from the Tri3, Tri5 and Tri7 genes of the trichothecene gene cluster was established for the identification of the different chemotypes among Fusarium graminearum, F. culmorum and F. cerealis. Using the selected primers, specific amplification products of 625, 354 and 708 bp were obtained from Fusarium isolates producing nivalenol, 3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol, respectively. Moreover, the multiplex PCR was successfully used to identify the chemotype of the Fusarium species contaminating wheat kernels. Four picograms of fungal DNA were found to be necessary to obtain a visible amplification product.
由于这些次生代谢产物的毒性存在显著差异，因此快速区分镰刀菌属给定物种/种群中的单端孢霉烯化学型的能力很重要。基于从单端孢霉烯基因簇的Tri3、Tri5和Tri7基因衍生的引物对，建立了多重PCR检测方法，用于鉴定禾谷镰刀菌、燕麦镰刀菌和小麦镰刀菌中的不同化学型。使用选定的引物，分别从产生雪腐镰刀菌烯醇、3-乙酰脱氧雪腐镰刀菌烯醇和15-乙酰脱氧雪腐镰刀菌烯醇的镰刀菌分离物中获得了625、354和708 bp的特异性扩增产物。此外，多重PCR成功用于鉴定污染小麦籽粒的镰刀菌物种的化学型。发现获得可见扩增产物需要4皮克真菌DNA。
