Tung J S, O'Donnell P V, Fleissner E, Boyse E A
J Exp Med. 1978 Apr 1;147(4):1280-4. doi: 10.1084/jem.147.4.1280.
The family of glycoproteins called gp70 includes molecules that are the main constituent of murine C-type viral envelopes, and some that are expressed as mendelian constituents of thymocyte plasma membranes in the absence of virions. To investigate further the relation of viral gp70s to plasma- membrane gp70s we compared peptide maps of gp70s derived by immunoprecipitation from cells infected with chosen viruses and from various thymocytes and leukemiacells known to express one or more of three immunogenetically defined gp70 types: Glx-gp70, X-gp70, and O-gp70. Maps of gp70 from cultured cells infected with ecotropic and xenotropic viruses were distinguishable from one another, and in general resembled gp70 maps prepared directly from ecotropic and xenotropic virions respectively. Maps of gp70s immunoprecipitated from thymocytes of five mouse strains and from two A strain T-cell leukemias also fell into two distinguishable and generally corresponding patterns. Thus peptide-mapping substantiates earlier conclusions that viral gp70s and plasma-membrane gp70s inherited independently of virus-production are highly related or identical molecules. The gp70 maps of thymocytes from B6, B6-G(+IX), 129, and A mice formed a group resembling the map from cultured cells infected with xenotropic virus. Thymocytes from AKR mice, and the two A strain leukemias, gave gp70 maps conforming more to the second pattern, that of cultured cells infected with ecotropic virus. This second pattern probably comprises at least two gp70 types, one of which is X-gp70. Our data indicate that the G(IX)-gp70 and O-gp70 sub-species of gp70 expressed in the cell populations we have studied are coded by xenotropic viral genomes, and X-gp70 by ecotropic viral genomes.
被称为gp70的糖蛋白家族包括一些分子，它们是鼠C型病毒包膜的主要成分，还有一些在没有病毒体的情况下作为胸腺细胞质膜的孟德尔成分表达。为了进一步研究病毒gp70与质膜gp70之间的关系，我们比较了通过免疫沉淀从感染特定病毒的细胞以及已知表达三种免疫遗传学定义的gp70类型（Glx-gp70、X-gp70和O-gp70）中的一种或多种的各种胸腺细胞和白血病细胞中获得的gp70的肽图。来自嗜亲性和异嗜性病毒感染的培养细胞的gp70图彼此可区分，并且总体上分别类似于直接从嗜亲性和异嗜性病毒体制备的gp70图。从五种小鼠品系的胸腺细胞和两种A品系T细胞白血病中免疫沉淀的gp70图也分为两种可区分且总体上相对应的模式。因此，肽图分析证实了早期的结论，即独立于病毒产生而遗传的病毒gp70和质膜gp70是高度相关或相同的分子。来自B6、B6-G(+IX)、129和A小鼠的胸腺细胞的gp70图形成了一组，类似于来自异嗜性病毒感染的培养细胞的图。来自AKR小鼠的胸腺细胞以及两种A品系白血病给出的gp70图更符合第二种模式，即嗜亲性病毒感染的培养细胞的模式。这第二种模式可能至少包括两种gp70类型，其中一种是X-gp70。我们的数据表明，在我们研究的细胞群体中表达的gp70的G(IX)-gp70和O-gp70亚类由异嗜性病毒基因组编码，而X-gp70由嗜亲性病毒基因组编码。
