一种改良的克里斯滕森尿素和CLSI肉汤微量稀释法，用于检测六种马拉色菌属菌种对伏立康唑、伊曲康唑和酮康唑的敏感性。
A modified Christensen's urea and CLSI broth microdilution method for testing susceptibilities of six Malassezia species to voriconazole, itraconazole, and ketoconazole.
作者信息
Rincón S, Cepero de García M C, Espinel-Ingroff A
机构信息
Centro De Investigactiones Microbiológicas CIMIC, Universidad de los Andes, Bogotá, Columbia.
出版信息
J Clin Microbiol. 2006 Sep;44(9):3429-31. doi: 10.1128/JCM.00989-06.
Abstract
Two supplemented broths (Christensen's urea with 0.1% Tween 80 and 0.5% Tween 40 and RPMI 1640 with 1% glycerol, 1% peptone, 1.8% glucose, and 0.05% Tween 80) were evaluated to determine voriconazole, itraconazole, and ketoconazole MICs for 200 Malassezia sp. isolates. Malassezia globosa and M. restricta were the least susceptible species (MICs at which 90% of the isolates tested were inhibited, 1 to >or=8 microg/ml versus 0.25 to 1 microg/ml).
摘要
评估了两种补充肉汤（含0.1%吐温80和0.5%吐温40的克里斯滕森尿素肉汤以及含1%甘油、1%蛋白胨、1.8%葡萄糖和0.05%吐温80的RPMI 1640培养基），以确定200株马拉色菌属分离株对伏立康唑、伊曲康唑和酮康唑的最低抑菌浓度（MIC）。球形马拉色菌和限制性马拉色菌是最不易感的菌种（90%受试分离株被抑制时的MIC值分别为1至≥8μg/ml对比0.25至1μg/ml）。
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