Chovanová Zuzana, Muchová Jana, Sivonová Monika, Dvoráková Monika, Zitnanová Ingrid, Waczulíková Iveta, Trebatická Jana, Skodácek Igor, Duracková Zdenka
Department of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova, Bratislava, Slovak Republic.
Free Radic Res. 2006 Sep;40(9):1003-10. doi: 10.1080/10715760600824902.
The purpose of this randomized, double-blind and placebo controlled study was to test the effect of polyphenolic extract of pine bark Pycnogenol (Pyc) on the level of oxidized purines represented by 8-oxo-7,8-dihydroguanine (8-oxoG) and on the total antioxidant status (TAS) in children with attention deficit/hyperactivity disorder (ADHD).We have found significantly increased damage to DNA in ADHD children when compared to controls. 8-oxoG was significantly lower after 1 month of Pyc administration in comparison to the beginning state and to placebo group. TAS in ADHD children was lower in comparison to controls. After Pyc administration, TAS was elevated but statistically significant increase was recorded after 1 month of termination of Pyc application. Improvement of DNA damage and TAS after Pyc administration is associated with the improvement of attention in ADHD children. In conclusion, Pycnogenol(R) administration reduces oxidative damage to DNA, normalizes TAS and improves attention of ADHD children. Explanation of mutual relation between oxidative damage to DNA, TAS and symptoms of ADHD and mechanism of Pyc's action needs further investigations.
这项随机、双盲、安慰剂对照研究的目的是测试松树皮碧萝芷（Pyc）多酚提取物对以8-氧代-7,8-二氢鸟嘌呤（8-氧代鸟嘌呤，8-oxoG）为代表的氧化嘌呤水平以及对注意缺陷多动障碍（ADHD）儿童总抗氧化状态（TAS）的影响。我们发现，与对照组相比，ADHD儿童的DNA损伤显著增加。与初始状态及安慰剂组相比，服用Pyc 1个月后8-氧代鸟嘌呤显著降低。与对照组相比，ADHD儿童的TAS较低。服用Pyc后，TAS有所升高，但在停用Pyc 1个月后才记录到有统计学意义的显著升高。服用Pyc后DNA损伤和TAS的改善与ADHD儿童注意力的改善相关。总之，服用碧萝芷（Pycnogenol®）可减少对DNA的氧化损伤，使TAS正常化，并改善ADHD儿童的注意力。对DNA氧化损伤、TAS与ADHD症状之间的相互关系以及Pyc作用机制的解释需要进一步研究。
