Hutchinson H D, Ziegler D W, Feorino P M
J Clin Microbiol. 1975 May;1(5):429-33. doi: 10.1128/jcm.1.5.429-433.1975.
A rapid microradioimmunoassay (RIA) technique was adapted for quantitatively measuring antibody titers to antigens occurring in Epstein-Barr virus (EBV)-infected lymphoid cells. In these experiments two EBV-infected cell lines, HR1K and EB-3, were used as antigen-positive cells and Molt-4 was used as the negative control cells. The antibody titers of sera from suspected infectious mononucleosis patients were compared by RIA and indirect fluorescent antibody (IFA) methods. As determined by each of the methods, 14 of 19 sera had positive antibody titers and the remainder of the sera had negative antibody titers. Thus, the two methods agreed completely in differentiating sera with antibodies to EBV antigens. To further evaluate the antibody specificity of the RIA, the antibody titers of paired sera, pre- or early infection and postinfection, from five confirmed infectious mononucleosis patients were determined by RIA and IFA. Seroconversion was demonstrated by both RIA and IFA for each of the patients. Thus, the sensitivity and specificity of the two procedures are about the same.
一种快速微量放射免疫测定（RIA）技术被用于定量测量针对爱泼斯坦-巴尔病毒（EBV）感染的淋巴细胞中出现的抗原的抗体滴度。在这些实验中，两种EBV感染的细胞系HR1K和EB-3被用作抗原阳性细胞，而Molt-4被用作阴性对照细胞。通过RIA和间接荧光抗体（IFA）方法比较了疑似传染性单核细胞增多症患者血清的抗体滴度。通过每种方法测定，19份血清中有14份抗体滴度为阳性，其余血清抗体滴度为阴性。因此，这两种方法在区分具有针对EBV抗原抗体的血清方面完全一致。为了进一步评估RIA的抗体特异性，通过RIA和IFA测定了5名确诊的传染性单核细胞增多症患者感染前或感染早期及感染后配对血清的抗体滴度。两种方法均证明每位患者都发生了血清转化。因此，这两种检测方法的灵敏度和特异性大致相同。
