爱泼斯坦 - 巴尔病毒DNA的复性动力学：与哺乳动物DNA的非同源性以及不同疾病中病毒DNA的同源性
Reassociation kinetics for Epstein-Barr virus DNA: nonhomology to mammalian DNA and homology of viral DNA in various diseases.
作者信息
Kawai Y, Nonoyama M, Pagano J S
出版信息
J Virol. 1973 Nov;12(5):1006-12. doi: 10.1128/JVI.12.5.1006-1012.1973.
Abstract
The number of Epstein-Barr virus (EBV) genomes per cell in established leukocytic lines and tissue specimens has been evaluated by measuring DNA-DNA reassociation kinetics with hydroxyapatite chromatography. Under the proper conditions, this method is sufficiently sensitive to detect EBV DNA in the amount of 0.1 genome per cell. All the samples tested that have been suspected to be without EBV DNA by cRNA hybridization proved negative by this more sensitive specific analysis. These included Hela and Hep2 cells, a negative case of Burkitt's lymphoma, two negative cases of nasopharyngeal carcinoma, and two established human leukocytic lines. Homology tests conducted with single-strand-specific nuclease S1 indicated that the viral DNA from a nasopharyngeal carcinoma and infectious mononucleosis were more than 90% homologous to EBV DNA.
摘要
通过用羟基磷灰石色谱法测量DNA-DNA重缔合动力学，对已建立的白细胞系和组织标本中每个细胞的爱泼斯坦-巴尔病毒（EBV）基因组数量进行了评估。在适当条件下，该方法灵敏度足够高，能够检测到每个细胞中0.1个基因组的EBV DNA。所有经cRNA杂交怀疑无EBV DNA的测试样本，经这种更灵敏的特异性分析均呈阴性。这些样本包括Hela和Hep2细胞、1例伯基特淋巴瘤阴性病例、2例鼻咽癌阴性病例以及2个已建立的人白细胞系。用单链特异性核酸酶S1进行的同源性测试表明，来自鼻咽癌和传染性单核细胞增多症的病毒DNA与EBV DNA的同源性超过90%。
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Homology between Epstein-Barr virus DNA and viral DNA from Burkitt's lymphoma and nasopharyngeal carcinoma determined by DNA-DNA reassociation kinetics.通过DNA-DNA重缔合动力学测定爱泼斯坦-巴尔病毒DNA与伯基特淋巴瘤和鼻咽癌病毒DNA之间的同源性。
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