Gustmann C, Altmannsberger M, Osborn M, Griesser H, Feller A C
Department of Pathology, University of Giessen, Federal Republic of Germany.
Am J Pathol. 1991 Jun;138(6):1413-22.
The immunophenotypes of 74 malignant lymphomas (9 Hodgkin's disease, 19 low-grade B-cell, 20 high-grade B-cell, 8 T-cell, and 18 large cell anaplastic lymphomas [LCAL]) have been characterized with antibodies against leucocyte differentiation antigens, keratin, and vimentin. All the non-LCAL were CD45 positive and keratin negative. The LCALs had a more varied immunophenotype, with CD45 present only in 11 of 18 cases and keratin present in 5 of 18 of these rare lymphomas. The lymphoid origin of these latter cases was proven by gene rearrangement studies. All LCALs were CD30+, and, where tested, vimentin positive. Of four different vimentin monoclonal antibodies tested, V9 and MVI stained the highest number of lymphomas. Positive staining of tumor cells was seen in 61 of 71 cases. Vimentin-negative cases included Burkitt's as well as some follicular lymphomas.
利用抗白细胞分化抗原、角蛋白和波形蛋白的抗体对74例恶性淋巴瘤（9例霍奇金病、19例低度B细胞淋巴瘤、20例高度B细胞淋巴瘤、8例T细胞淋巴瘤和18例间变性大细胞淋巴瘤[LCAL]）的免疫表型进行了特征分析。所有非LCAL均为CD45阳性且角蛋白阴性。LCAL的免疫表型更为多样，18例中仅有11例CD45阳性，在这些罕见淋巴瘤的18例中有5例角蛋白阳性。通过基因重排研究证实了后一类病例的淋巴起源。所有LCAL均为CD30阳性，经检测波形蛋白也呈阳性。在检测的4种不同波形蛋白单克隆抗体中，V9和MVI染色的淋巴瘤数量最多。71例中有61例肿瘤细胞呈阳性染色。波形蛋白阴性的病例包括伯基特淋巴瘤以及一些滤泡性淋巴瘤。
