Faulkner Amanda E, Kim Moon K, Iuvone P Michael, Pardue Machelle T
Rehab R&D Center, Atlanta VAMC, Decatur, GA, USA.
J Neurosci Methods. 2007 Mar 30;161(1):96-100. doi: 10.1016/j.jneumeth.2006.10.011. Epub 2006 Nov 28.
Recently a murine model has been developed for use in form deprivation myopia experiments. Due to the small size of the head and eye, methods to blur visual input to the mouse eye are challenging. Previous methods to induce form deprivation include lid suture and gluing diffuser goggles directly to the fur around the eye. In this paper we describe a new method of goggling using a head pedestal and goggle, which improves compliance and allows for better ocular health. Nob mice, previously shown to be highly susceptible to form deprivation myopia, were used for these experiments. Immediately following baseline refraction using an infrared automated photorefractor, mice were either goggled with a diffuser attached directly to the fur or with a head-mounted goggling apparatus. The goggle apparatus consists of five main components: goggle and frame, head pedestal, acrylic cube for stabilization, and balancing bar. Mice were goggled for 2 weeks in which ocular health and goggle position was monitored and then had a final refraction. The use of head-mounted goggles resulted in 75% fewer instances of goggle loss and 55% fewer ocular complications compared to goggles glued to the fur. Both goggling methods induced a myopic shift of approximately 5 diopters. The head-mounted goggle apparatus provides an improved method for inducing form deprivation in mice and offers the ability to easily take repeated refractive measurements as well as allowing for the use of defocusing lenses.
最近开发了一种用于形觉剥夺性近视实验的小鼠模型。由于小鼠头部和眼睛尺寸较小，使小鼠眼睛视觉输入模糊的方法具有挑战性。以往诱导形觉剥夺的方法包括眼睑缝合和将扩散器护目镜直接粘贴在眼睛周围的毛发上。在本文中，我们描述了一种使用头部基座和护目镜的新的护目镜佩戴方法，该方法提高了依从性并有助于保持更好的眼部健康。此前已证明Nob小鼠对形觉剥夺性近视高度敏感，因此使用这些小鼠进行实验。在使用红外自动验光仪进行基线验光后，立即将小鼠佩戴直接粘贴在毛发上带有扩散器的护目镜或佩戴头戴式护目镜装置。护目镜装置由五个主要部件组成：护目镜和框架、头部基座、用于稳定的丙烯酸立方体和平衡杆。小鼠佩戴护目镜2周，在此期间监测眼部健康和护目镜位置，然后进行最终验光。与粘贴在毛发上的护目镜相比，使用头戴式护目镜导致护目镜丢失的情况减少了75%，眼部并发症减少了55%。两种护目镜佩戴方法均诱导了约5屈光度的近视偏移。头戴式护目镜装置为在小鼠中诱导形觉剥夺提供了一种改进的方法，并提供了轻松进行重复验光测量的能力，以及允许使用散焦镜片。
