Krause Trisha A, Liewehr Frederick R, Hahn Chin-Lo
Virginia Commonwealth University, Richmond, Virginia, USA.
J Endod. 2007 Jan;33(1):28-30. doi: 10.1016/j.joen.2006.08.011. Epub 2006 Oct 27.
This study compared the antimicrobial effect of MTAD, two of its components, doxycycline and citric acid, and sodium hypochlorite (NaOCl) in two in vitro models on Enterococcus faecalis. In the bovine tooth model, the lumens of 30 bovine dentin discs were infected with E. faecalis for 2 weeks before treating with either one of the experimental irrigants or saline. Bacteria in the shavings were collected with two sizes of burs and enumerated after overnight culturing. Zones of inhibition were recorded in the agar diffusion model for each irrigant. In the tooth model, NaOCl and doxycycline were more effective than control in killing E. faecalis at the shallow bur depth, but at the deeper bur depth only NaOCl was superior. In the agar diffusion model, NaOCl produced less inhibition than MTAD or doxycycline.
本研究在两种体外模型中比较了MTAD、其两种成分（强力霉素和柠檬酸）以及次氯酸钠（NaOCl）对粪肠球菌的抗菌效果。在牛牙模型中，30个牛牙本质盘的管腔在感染粪肠球菌2周后，再用任何一种实验冲洗剂或生理盐水进行处理。用两种尺寸的车针收集碎屑中的细菌，过夜培养后进行计数。在琼脂扩散模型中记录每种冲洗剂的抑菌圈。在牙模型中，在浅车针深度处，NaOCl和强力霉素在杀灭粪肠球菌方面比对照组更有效，但在较深车针深度处，只有NaOCl更具优势。在琼脂扩散模型中，NaOCl产生的抑制作用比MTAD或强力霉素小。
