Rathlev T
Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136/sti.51.5.296.
Motility of pathogenic T. pallidum was maintained in aerobic in vitro cultures for several weeks using a special medium. The latter consisted of McCoy's 5a medium supplemented with glutathione, sodium pyruvate, HEPES buffer, gentamycin (garamycin), and fetal calf serum. The virulence of the organisms was lost in 5 to 6 days. No multiplication of the organisms was observed. Four antibiotics (viomycin, kanamycin, gentamycin (garamycin), and neomycin) were tested for their bactericidal action and possible toxicity to T. pallidum. Gentamycin proved to be superior to the other three antibiotics in being non-toxic to the treponemes and showing a possible stimulatory effect on their motility and longevity. Cultivation of T. pallidum in cultured cells in the presence of the enzymes, superoxide dismutase and catalase, in a special medium showed possibilities for future experimentation under monitored, reduced oxygen pressure with a continuous system to dismutate superoxide radicals.
使用一种特殊培养基，致病性梅毒螺旋体在需氧体外培养中可保持运动能力数周。该培养基由添加了谷胱甘肽、丙酮酸钠、HEPES缓冲液、庆大霉素（加里霉素）和胎牛血清的 McCoy's 5a 培养基组成。这些微生物的毒力在 5 至 6 天内丧失。未观察到微生物的增殖。测试了四种抗生素（紫霉素、卡那霉素、庆大霉素（加里霉素）和新霉素）对梅毒螺旋体的杀菌作用和可能的毒性。事实证明，庆大霉素在对密螺旋体无毒且对其运动能力和存活时间可能具有刺激作用方面优于其他三种抗生素。在特殊培养基中，在超氧化物歧化酶和过氧化氢酶存在的情况下，在培养细胞中培养梅毒螺旋体，显示了未来在监测的、降低的氧气压力下，通过连续系统使超氧自由基歧化进行实验的可能性。
